Description

O'-(Carboxymethyl)Fluoresceinamide is a chemically modified, fluorescent labeling reagent derived from fluorescein, featuring a reactive carboxymethyl group. This compound is essential for researchers and manufacturers requiring a stable, high-efficiency fluorescent tag for biomolecule conjugation, particularly in aqueous environments. It is primarily used by professionals in the life sciences, diagnostics, and pharmaceutical development sectors for applications such as antibody labeling, protein tracking, and assay development.

Application

• Bioconjugation and Labeling: Used as a fluorescent probe for covalently labeling antibodies, proteins, peptides, and other biomolecules via its reactive carboxyl group.
• Diagnostic Assay Development: Serves as a key component in the creation of fluorescent immunoassays (FIAs), lateral flow tests, and other diagnostic kits.
• Cellular Imaging and Tracking: Enables the visualization and tracking of biomolecules within live or fixed cells for research in cell biology and pharmacology.
• Flow Cytometry Reagents: Incorporated into fluorescent antibody conjugates and other reagents used for cell analysis and sorting.
• Biomolecular Interaction Studies: Facilitates Fluorescence Resonance Energy Transfer (FRET) and other proximity-based assays to study protein-protein interactions.
• Sensor and Probe Fabrication: Utilized in the synthesis of advanced chemical sensors and molecular probes for detecting specific analytes.

Basic Information

Product Name	O'-(Carboxymethyl)Fluoresceinamide
CAS No.	442151-50-0
Molecular Formula	C24H17NO7
Molecular Weight	431.40 g/mol
Synonyms	5(6)-Carboxyfluorescein succinimidyl ester (alternative isomer); CMF; O-Carboxymethyl fluorescein amine; Fluorescein-O-carboxymethylamide; 6-Carboxyfluorescein N-succinimidyl ester derivative; 5-Carboxyfluorescein, SE; 5(6)-FAM, SE; N-Succinimidyl ester of carboxyfluorescein
EINECS	Contact for details

Quality Control

Our O'-(Carboxymethyl)Fluoresceinamide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light). For long-term storage, desiccate at -20°C.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λ max)	~492 nm
Emission (λ max)	~518 nm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.