Description

Cy7.5 Diacid is a near-infrared (NIR) fluorescent dye derivative featuring two carboxylic acid functional groups, enabling covalent conjugation to biomolecules. This compound is essential for creating highly sensitive and stable fluorescent probes for deep-tissue imaging and multiplexed detection. It is primarily needed by researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors for advanced bioanalytical applications.

Application

• Bio-conjugation and Probe Synthesis: The diacid groups serve as reactive handles for coupling to amines on antibodies, peptides, proteins, and nanoparticles.
• In Vivo Imaging: Used to label targeting agents for non-invasive, deep-tissue fluorescence imaging in preclinical research (e.g., cancer, vascular studies).
• Diagnostic Assay Development: A key component in developing high-sensitivity lateral flow assays (LFAs), ELISA, and other immunoassays requiring NIR detection.
• Flow Cytometry: Conjugated to antibodies or other ligands for multiplexed cell surface marker analysis using NIR channels.
• Material Science: Incorporation into polymers or coatings to create smart materials with fluorescent properties.
• DNA/RNA Labeling: After activation, can be used to label oligonucleotides for genetic analysis and fluorescence in situ hybridization (FISH).

Basic Information

Product Name	Cy7.5 Diacid
CAS No.	432491-45-7
Molecular Formula	C44H49N2O6S2+ • 2X- (X = counterion)
Molecular Weight	~765.99 (for free acid form, excluding counterions)
Synonyms	Cy7.5 Carboxylic Acid; Sulfo-Cyanine7.5 diacid; 2-[7-[1,3-Dihydro-1,1-dimethyl-3-(4-sulfobutyl)-2H-benz[e]indol-2-ylidene]-1,3,5-heptatrienyl]-1,1-dimethyl-3-(4-carboxybutyl)-1H-benz[e]indolium Hydroxide, Inner Salt; NIR-783 Diacid; Cyanine7.5 bis-carboxylic acid; Benz[e]indolium Cy7.5 derivative
EINECS	Contact for details

Quality Control

Our Cy7.5 Diacid is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry to verify optical properties. A comprehensive Certificate of Analysis (COA) detailing identity, purity (typically >95%), and performance specifications is provided with every shipment to ensure it meets the exacting standards of life science research and diagnostics.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store the lyophilized solid in a freezer. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue-black solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion pattern
Purity (HPLC)	≥ 95%
Absorption (λmax)	~788 nm ± 5 nm
Emission (λmax)	~808 nm ± 5 nm
Extinction Coefficient (ε)	≥ 200,000 cm-1M-1
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, DMF, water (basic pH)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.