Description

n-Acetyl-2'-o-[(1,1-Dimethylethyl)Dimethylsilyl]Cytidine is a key protected nucleoside intermediate, specifically designed for advanced oligonucleotide synthesis. This compound matters because it provides a critical, reactive building block for the precise construction of RNA and DNA analogs, enabling the development of novel therapeutics and research tools. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and academic laboratories focused on antisense oligonucleotides, siRNA, and other nucleic acid-based modalities.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): A crucial phosphoramidite precursor for the automated synthesis of modified RNA strands.
• Antisense Oligonucleotide (ASO) Development: Used in the research and production of therapeutic ASOs targeting genetic diseases.
• Small Interfering RNA (siRNA) Manufacturing: Serves as a protected monomer for constructing siRNA duplexes with enhanced stability and specificity.
• Research & Diagnostic Probe Synthesis: Enables the creation of labeled and modified nucleic acid probes for genomics and molecular diagnostics.
• Pharmaceutical Intermediate: A high-purity building block in the multi-step synthesis of active pharmaceutical ingredients (APIs) for nucleic acid therapies.
• Academic & Contract Research: Supports fundamental research in medicinal chemistry, biochemistry, and molecular biology requiring custom oligonucleotides.

Basic Information

Product Name	n-Acetyl-2'-o-[(1,1-Dimethylethyl)Dimethylsilyl]Cytidine
CAS No.	401812-97-3
Molecular Formula	C18H31N3O6Si
Molecular Weight	413.55 g/mol
Synonyms	2'-O-TBDMS-N4-acetylcytidine; N4-Acetyl-2'-O-(tert-butyldimethylsilyl)cytidine; 2'-O-(tert-Butyldimethylsilyl)-N4-acetylcytidine; 1-[(2R,3R,4R,5R)-5-[(tert-Butyldimethylsilyl)oxy]-3,4-dihydroxyoxolan-2-yl]-4-(acetylamino)pyrimidin-2-one; TBDMS-N4-Ac-cytidine; Protected Cytidine Derivative; Cytidine, N-acetyl-2'-O-[(1,1-dimethylethyl)dimethylsilyl]-
EINECS	Contact for details

Quality Control

Our n-Acetyl-2'-o-[(1,1-Dimethylethyl)Dimethylsilyl]Cytidine is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive oligonucleotide synthesis. We employ advanced analytical techniques, including HPLC, NMR, and mass spectrometry, to verify identity, purity, and the absence of critical impurities. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This product is hygroscopic (moisture-sensitive); containers must be kept tightly sealed and under an inert atmosphere (e.g., nitrogen or argon) after opening to prevent degradation. Keep away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.