Description

Dapi CAS NO 47165-04-8 is a high-purity, fluorescent dye widely recognized for its strong binding affinity to adenine-thymine (A-T) rich regions in double-stranded DNA. This compound is essential for advanced cellular and molecular biology research, providing a reliable tool for nuclear staining and chromosome visualization. It is a critical reagent for life science researchers, diagnostic kit manufacturers, and pharmaceutical companies engaged in fluorescence microscopy, flow cytometry, and high-content screening applications.

Application

• Fluorescent Nuclear Staining in fixed and live cells for microscopy.
• Chromosome Counterstaining in fluorescence in situ hybridization (FISH) techniques.
• Apoptosis Detection and cell viability assays.
• DNA Quantification and cell cycle analysis via flow cytometry.
• High-Content Screening (HCS) in drug discovery and toxicology studies.
• Microbiological Research for visualizing bacterial and fungal DNA.
• Diagnostic Reagent Component in kits for pathology and cytogenetics.

Basic Information

Product Name	Dapi
CAS No.	47165-04-8
Molecular Formula	C16H15N5·2HCl
Molecular Weight	350.25 g/mol (as free base)
Synonyms	4',6-Diamidino-2-phenylindole dihydrochloride; DAPI dihydrochloride; Dihydrochloride, 4',6-Diamidino-2-phenylindole; DAPI; D 9542; D 8417; 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; NSC 106319
EINECS	Contact for details

Quality Control

Our Dapi is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopy for identity confirmation, and stringent controls for fluorescence performance. Certificates of Analysis (COA) detailing purity, absorbance/emission profiles, and bioburden are provided to ensure the material meets the exacting standards required for research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 5.0%
Absorbance (Max)	~358 nm
Fluorescence (Max)	~461 nm (when bound to DNA)
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.