Description

Tetranitro Blue Tetrazolium is a specialized tetrazolium salt derivative, widely recognized for its role as a redox indicator and chromogenic substrate in biochemical assays. Its primary value lies in its ability to produce a highly colored, insoluble formazan dye upon reduction, making it a critical reagent for detecting dehydrogenase activity and cell viability. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors, where precise enzymatic and cellular analysis is paramount.

Application

• As a chromogenic substrate in histochemical staining for the localization of dehydrogenase enzymes in tissue sections.
• Critical component in cell viability and cytotoxicity assays (e.g., MTT assay alternatives) for pharmaceutical screening.
• Used as an electron acceptor in biochemical research to study mitochondrial activity and respiratory chain function.
• Employed in microbiology for the detection of microbial dehydrogenase activity.
• Application in plant physiology research to assess seed viability and root activity.
• Utilized in diagnostic kit formulations for clinical biochemistry.

Basic Information

Product Name	Tetranitro Blue Tetrazolium
CAS No.	42798-98-1
Molecular Formula	C40H30N10O6
Molecular Weight	746.74 g/mol
Synonyms	Tetranitro BT; TNBT; Tetranitrotetrazolium Blue Chloride; 2,2',5,5'-Tetraphenyl-3,3'-(p-diphenylene)ditetrazolium chloride, tetranitro derivative; Blue Tetrazolium, Tetranitro; Nitro BT; Tetrazolium Blue, Tetranitro; TNBT Chloride
EINECS	Contact for details

Quality Control

Our Tetranitro Blue Tetrazolium is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR), to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term stability, consider storage under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.