Description

4-Hydroxycyclophosphamide is a key intermediate metabolite in the bioactivation pathway of the prodrug cyclophosphamide. This compound matters significantly for researchers and manufacturers focused on oncology drug development and metabolism studies, as it is directly responsible for the alkylating activity and cytotoxic effects. It is primarily needed by pharmaceutical R&D laboratories, academic institutions, and fine chemical suppliers serving the anticancer therapeutic and biochemical research sectors.

Application

• Pharmaceutical Reference Standard: Used as a high-purity analytical standard for quantifying cyclophosphamide and its metabolites in pharmacokinetic and bioequivalence studies.
• Oncology Research: Critical for in vitro and in vivo studies investigating the mechanism of action, metabolic activation, and resistance profiles of cyclophosphamide-based therapies.
• Metabolite Synthesis: Serves as a vital synthetic intermediate for the preparation of other cyclophosphamide metabolites and related chemical probes.
• Biochemical Assay Development: Employed in the development and validation of enzymatic assays and cell-based tests to study drug metabolism and toxicity.
• Drug Discovery & Development: Used in early-stage discovery to understand structure-activity relationships (SAR) and to design next-generation prodrugs.
• Toxicology Studies: Applied in research to elucidate the specific toxicological pathways and side effects associated with cyclophosphamide therapy.

Basic Information

Product Name	4-Hydroxycyclophosphamide
CAS No.	40277-05-2
Molecular Formula	C7H15Cl2N2O2P
Molecular Weight	261.09 g/mol
Synonyms	4-Hydroxycyclophosphamide; 4-Hydroxy-CPA; 2-[Bis(2-chloroethyl)amino]tetrahydro-2H-1,3,2-oxazaphosphorine 4-oxide; Cyclophosphamide Metabolite; 4-OH-CPA; NSC-26271; Aldophosphamide (tautomer); 4-Hydroxy-N,N-bis(2-chloroethyl)tetrahydro-2H-1,3,2-oxazaphosphorin-2-amine 4-oxide
EINECS	Contact for details

Quality Control

Our 4-Hydroxycyclophosphamide is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency, suitable for research and development applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) due to its sensitivity to oxidation and moisture. Allow the sealed container to reach room temperature before opening to minimize condensation and degradation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Individual impurity ≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.