Description

Xylene Cyanol Ff is a high-purity triphenylmethane dye widely used as a tracking dye in molecular biology and biochemistry. This compound, with CAS number 4463-44-9, is valued for its distinct blue color and predictable migration properties in gel electrophoresis. It is an essential laboratory reagent for researchers and technicians requiring precise visualization of nucleic acid samples during separation processes. Key industrial users include manufacturers of diagnostic kits, life science research consumables, and analytical laboratories.

Application

• Gel Electrophoresis Tracking Dye: Primarily used as a visible front-running marker in agarose and polyacrylamide gel electrophoresis (PAGE) for nucleic acids (DNA/RNA).
• Molecular Biology Reagents: A critical component in loading buffers and sample preparation kits for DNA sequencing, PCR, and other nucleic acid analysis techniques.
• Biochemical Research: Employed in laboratory research to monitor the progress of electrophoretic runs and estimate fragment sizes.
• Diagnostic Manufacturing: Used in the production of in-vitro diagnostic (IVD) kits and devices that utilize electrophoresis for clinical analysis.
• Dye Marker for Chromatography: Can serve as a colored marker in certain column chromatography techniques to visualize flow.
• Teaching & Academic Labs: A standard reagent in educational settings for demonstrating fundamental biochemistry and genetics techniques.

Basic Information

Product Name	Xylene Cyanol Ff
CAS No.	4463-44-9
Molecular Formula	C25H27N2NaO6S2
Molecular Weight	538.61 g/mol
Synonyms	Acid Blue 147; C.I. 42135; Xylene Cyanol FF; Xylene Cyanol; Xylene Cyanol FCF; Brilliant Acid Blue BS; L-Guronsaure, 5-(acetylamino)-4,7,8,9-tetrahydro-3,5-dihydroxy-9,10-dihydro-4,7,10-trioxo-2H-naphtho[2,3-b]pyran-2-yl ester; Sodium 4-[[4-(dimethylamino)phenyl][4-(ethylamino)-1-naphthyl]methylene]-3-oxo-2,5-cyclohexadien-1-ylidene]dimethylammonium hydroxide inner salt, sulfonated derivative.
EINECS	224-714-1

Quality Control

Our Xylene Cyanol Ff is manufactured under a strict quality management system to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometric analysis for absorbance characteristics, and identity confirmation (IR). Certificates of Analysis (COA) detailing all test results are provided to guarantee the material meets the stringent requirements of life science research and in-vitro diagnostic manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep the container tightly sealed to protect from moisture. Store away from strong oxidizing agents.

Specification

Item	Specification
Appearance	Dark blue to greenish-blue powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
λmax (Absorbance)	610 - 630 nm
Loss on Drying	≤ 5.0 %
Solubility (in Water)	Clear, blue solution
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.