Description

Tetramethylrhodamine Isothiocyanate (TRITC) is a highly reactive, fluorescent labeling reagent widely used in life science research. Its core value lies in its ability to form stable thiourea bonds with primary amines, enabling the specific tagging of proteins, antibodies, and other biomolecules for detection and imaging. This product is essential for researchers and manufacturers in the fields of flow cytometry, fluorescence microscopy, immunoassay development, and nucleic acid analysis.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amine groups (e.g., lysine residues) for use in immunofluorescence (IF) and immunohistochemistry (IHC).
• Cell and Tissue Staining: Direct or indirect labeling for visualization in fluorescence microscopy and confocal imaging.
• Flow Cytometry: Preparation of fluorescent antibody conjugates for cell surface and intracellular marker detection.
• Nucleic Acid Labeling: Tagging of oligonucleotide probes for fluorescence in situ hybridization (FISH) and other hybridization techniques.
• Bioconjugation and Probe Development: Synthesis of custom fluorescent probes for receptor binding studies, enzyme assays, and diagnostic test kits.
• Material Science: Incorporation into polymers or surfaces to create fluorescent materials for tracking and sensing applications.

Basic Information

Product Name	Tetramethylrhodamine Isothiocyanate
CAS No.	4158-89-8
Molecular Formula	C25H21N3O3S
Molecular Weight	443.52 g/mol
Synonyms	TRITC; Tetramethylrhodamine-5-isothiocyanate; 5-TRITC; Rhodamine B isothiocyanate; 5-(and-6)-Tetramethylrhodamine isothiocyanate; Mixed isomers; Lissamine rhodamine B sulfonyl chloride; [5-(or 6)-Isothiocyanato]tetramethylrhodamine
EINECS	224-002-5

Quality Control

Our Tetramethylrhodamine Isothiocyanate is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure consistent performance and lot-to-lot reproducibility for critical research and diagnostic applications. Certificates of Analysis (COA) detailing purity, fluorescence properties, and other relevant specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccant or under an inert atmosphere.

Specification

Item	Specification
Appearance	Red to dark red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 90% (Mixed isomers)
Absorption (λmax)	~550 nm
Emission (λmax)	~575 nm
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Loss on Drying	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.