Description

2′,7′-Dichlorodihydrofluorescein Diacetate is a cell-permeable, non-fluorescent probe widely used as a highly sensitive indicator for reactive oxygen species (ROS) detection. Its core value lies in its ability to be oxidized by intracellular ROS to form the highly fluorescent dichlorofluorescein, enabling quantitative measurement of oxidative stress in biological systems. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors, particularly for applications in cell biology, toxicology, and drug discovery.

Application

• Intracellular Reactive Oxygen Species (ROS) Detection: Primary application for measuring oxidative stress in live cells.
• Pharmaceutical Screening: Used in high-throughput assays to evaluate the antioxidant or pro-oxidant effects of drug candidates.
• Toxicology Studies: Monitoring oxidative damage induced by environmental toxins or chemical agents in cellular models.
• Neuroscience Research: Investigating ROS involvement in neuronal cell death and neurodegenerative disease pathways.
• Immunology and Inflammation Research: Assessing ROS production in immune cells like macrophages and neutrophils during inflammatory responses.
• Apoptosis and Cell Viability Assays: Used as a component in multiplex assays to correlate ROS levels with cell health and death mechanisms.

Basic Information

Product Name	2′,7′-Dichlorodihydrofluorescein Diacetate
CAS No.	4091-99-0
Molecular Formula	C24H16Cl2O7
Molecular Weight	487.29 g/mol
Synonyms	DCFH-DA; 2',7'-Dichlorodihydrofluorescein diacetate; H2DCFDA; Dichlorofluorescin diacetate; 2',7'-Dichlorofluorescin diacetate; 6-Carboxy-2',7'-dichlorodihydrofluorescein diacetate; 2',7'-Dichlorofluorescein diacetate (reduced form); Reactive Oxygen Species Detection Probe
EINECS	Contact for details

Quality Control

Our 2′,7′-Dichlorodihydrofluorescein Diacetate is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccated conditions. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store in aliquots under an inert atmosphere to prevent oxidation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol
Fluorescence Properties	Ex/Em: ~495 nm/~529 nm (upon oxidation)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.