Description

Acridine Orange CAS NO 494-38-2 is a versatile metachromatic fluorescent dye and nucleic acid-selective cationic fluorophore. This compound is critical for its ability to differentially stain DNA and RNA, enabling vital applications in cell biology and diagnostic research. It is an essential reagent for researchers and quality control laboratories in the biotechnology, pharmaceutical, and life sciences sectors.

Application

• Fluorescent Staining in Cytometry: Used for cell cycle analysis, apoptosis detection, and viability assays in flow cytometry and microscopy.
• Nucleic Acid Detection: Selective staining of DNA (green fluorescence) and RNA (red fluorescence) in fixed cells and tissue sections.
• Microbiological Research: Employed as a fluorescent probe for the detection and enumeration of microorganisms in environmental and clinical samples.
• Lysosome and Autophagy Studies: Useful for staining acidic vesicular organelles (AVOs) like lysosomes and autophagosomes.
• Blood Cell Analysis: Applied in hematology for differential staining of leukocytes and reticulocytes.
• Material Science: Acts as a photosensitizer in photodynamic therapy research and in the development of light-activated materials.

Basic Information

Product Name	Acridine Orange
CAS No.	494-38-2
Molecular Formula	C17H19N3
Molecular Weight	265.36 g/mol
Synonyms	Acridine Orange Base; 3,6-Bis(dimethylamino)acridine; Basic Orange 14; C.I. 46005; Euchrysine 3R; Euchrysine G; Solvent Orange 15; Acridine Orange Free Base; 3,6-Bis(dimethylamino)acridine hydrochloride (for salt forms); AO
EINECS	207-795-2

Quality Control

Our Acridine Orange is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopy for identification, and tests for residual solvents and heavy metals. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, keep the container tightly sealed to minimize exposure to atmospheric moisture. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to brown crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 20 ppm
Fluorescence Properties	Conforms to reference spectrum

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.