Description

Hematein CAS NO 475-25-2 is a natural oxidation product of hematoxylin, widely recognized as a critical biological stain and histology dye. It serves as the active component in hematoxylin-based staining solutions, forming complexes with metal mordants like aluminum or iron to produce intense blue or black colors. This compound is essential for researchers and laboratories involved in histopathology, cytology, and life science research, enabling the clear visualization of cell nuclei and tissue structures under a microscope.

Application

• Histological Staining: Primary application as the active dye in hematoxylin solutions (e.g., Harris's hematoxylin, Mayer's hematoxylin) for staining cell nuclei in tissue sections.
• Cytological Analysis: Used in Papanicolaou (Pap) stains and other cytological procedures to differentiate nuclear material in cell smears.
• Microscopy & Diagnostics: A cornerstone reagent in pathology labs for cancer diagnosis, routine biopsy examination, and medical research.
• Biological Research: Employed in various laboratory protocols for chromosome staining, in-situ hybridization, and immunohistochemistry (IHC) as a counterstain.
• Educational & Teaching Kits: A standard component in biology and medical education kits for teaching microscopic anatomy.
• Industrial Dye Intermediate: Can serve as a precursor or intermediate in the synthesis of other specialized dyes and colorants.

Basic Information

Product Name	Hematein
CAS No.	475-25-2
Molecular Formula	C16H12O6
Molecular Weight	300.26 g/mol
Synonyms	Hematein; Hematine; 7,11b-Dihydroindeno[2,1-c]chromene-3,4,6a,9,10(6H)-pentol; 3,4,6a,9,10-Pentahydroxy-6H-indeno[2,1-c]chromen-6-one; Natural Black 1; CI 75290; Hematoxylin oxidation product; Haematine (British spelling)
EINECS	207-488-3

Quality Control

Our Hematein is produced and tested under a rigorous quality management system. Each batch is analyzed to ensure high purity and performance consistency for critical histological applications. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and related substance tests is provided with every shipment and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be stored away from direct light exposure to maintain stability.

Specification

Item	Specification
Appearance	Reddish-brown to dark brown powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility (Test)	Soluble in alkaline solutions and alcohol
Staining Performance	Passes test (vs. reference standard)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.