Description

5-Carboxy-X-Rhodamine n-Succinimidyl Ester is a high-purity, amine-reactive fluorescent labeling reagent essential for advanced bioconjugation. This compound matters because it provides a stable, bright red-emitting fluorophore for creating highly sensitive probes used in detection and imaging. It is critically needed by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors for applications such as flow cytometry, fluorescence microscopy, and immunoassay development.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides via primary amine groups.
• Flow Cytometry & Cell Sorting (FACS): Preparation of bright, photostable antibody-dye conjugates for cell surface marker analysis.
• Fluorescence Microscopy & Imaging: Synthesis of probes for fixed and live-cell imaging with excellent spectral properties.
• Immunoassay Development: Creation of fluorescent tracers and detection reagents for ELISA and other plate-based assays.
• Diagnostic Kit Manufacturing: Key raw material for producing in-vitro diagnostic (IVD) components.
• Nucleic Acid Labeling: Tagging of amine-modified DNA or RNA for genetic analysis and sequencing.
• Drug Discovery & High-Throughput Screening (HTS): Development of fluorescent probes for target engagement and binding studies.

Basic Information

Product Name	5-Carboxy-X-Rhodamine n-Succinimidyl Ester
CAS No.	344402-35-3
Molecular Formula	C34H31N3O9
Molecular Weight	625.63 g/mol
Synonyms	5-ROX NHS Ester; 5-Carboxy-X-rhodamine, succinimidyl ester; 6-Carboxy-X-rhodamine N-succinimidyl ester; X-Rhodamine-5-(and-6)-isomer, NHS ester; 5-Carboxy-X-rhodamine N-hydroxysuccinimide ester; ROX NHS Ester (5-isomer); 5-Carboxy-X-rhodamine, SE; 5-ROX, SE
EINECS	Contact for details

Quality Control

Every batch of our 5-Carboxy-X-Rhodamine n-Succinimidyl Ester is manufactured and tested under strict quality management systems. We guarantee high chemical purity and optimal performance for sensitive applications. Comprehensive analytical testing, including HPLC for purity and identity confirmation, is performed. A detailed Certificate of Analysis (COA) is provided with each shipment to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	575 - 585 nm
Emission (λmax)	600 - 610 nm
Extinction Coefficient (ε)	≥ 80,000 cm⁻¹M⁻¹
Solubility	Soluble in DMF, DMSO, acetonitrile
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.