Description

Fluo-3, Pentaammonium Salt is a high-purity, cell-permeant fluorescent calcium indicator essential for intracellular calcium ion (Ca²⁺) concentration measurement. Its core value lies in providing researchers with a highly sensitive and selective tool for real-time visualization of calcium signaling dynamics in live cells. This product is critically needed by scientists and R&D professionals in the fields of neuroscience, cardiovascular research, pharmacology, and cell biology for applications ranging from basic research to high-throughput drug screening.

Application

• Intracellular Calcium Imaging: Primary use for quantifying and visualizing real-time changes in cytosolic free Ca²⁺ concentrations in live cells using fluorescence microscopy or flow cytometry.
• Neuroscience Research: Studying calcium transients in neurons, synaptic plasticity, neurotransmitter release, and neuronal network activity.
• Cardiovascular Research: Monitoring calcium sparks and waves in cardiomyocytes to understand cardiac muscle contraction, arrhythmias, and drug effects.
• Drug Discovery & Toxicology Screening: Employed in high-content screening (HCS) assays to evaluate compound effects on cellular calcium homeostasis and signaling pathways.
• Cell Signaling Studies: Investigating GPCR (G-protein coupled receptor) activation, ion channel function, and second messenger systems.
• Physiology & Pathophysiology Research: Exploring the role of calcium in processes like muscle contraction, secretion, apoptosis, and in disease models.

Basic Information

Product Name	Fluo-3, Pentaammonium Salt
CAS No.	339221-91-9
Molecular Formula	C₅₁H₅₀N₅O₂₃ • 5NH₃ (or commonly represented as C₅₁H₅₀N₅O₂₃ • 5NH₃)
Molecular Weight	~1129.94 g/mol (Free acid), Pentaammonium salt form weight available on request.
Synonyms	Fluo-3 pentaammonium salt; Fluo-3, AM; Fluo-3 acetoxymethyl ester (note: AM ester is a common derivative); 2-[2-[2-[Bis(carboxymethyl)amino]-5-(2,7-dichloro-6-hydroxy-3-oxo-9-xanthenyl)phenoxy]ethoxy]-4-methylphenylcarbamoylmethyl-iminodiacetic acid pentaammonium salt; Calcium indicator Fluo-3; Fluo3; Fluo 3.
EINECS	Contact for details.

Quality Control

Our Fluo-3, Pentaammonium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and performance reliability for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC purity analysis, fluorescence spectroscopy validation, and functional testing in cell-based assays. Comprehensive Certificates of Analysis (COA) detailing purity, identity, and performance characteristics are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under inert atmosphere or in a vacuum desiccator. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Anhydrous basis)
Water Content (KF)	≤ 5.0 %
Fluorescence Properties	Ex/Em ~506/526 nm (bound to Ca²⁺); Validated performance in standard buffer
Functional Activity (Cell Assay)	Produces robust calcium signal upon stimulation in validated cell lines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.