Description

Bodipy Cyclopamine CAS NO 334658-24-1 is a fluorescently labeled derivative of the Hedgehog signaling pathway inhibitor, cyclopamine. This compound is specifically designed for advanced research applications, combining the potent biological activity of cyclopamine with the BODIPY fluorophore for detection and visualization. It is an essential tool for scientists in pharmaceutical discovery, cancer research, and developmental biology who require precise tracking of inhibitor localization and mechanism of action studies.

Application

• Fluorescence Microscopy & Cellular Imaging: Used as a fluorescent probe to visualize the uptake, localization, and trafficking of cyclopamine within live or fixed cells.
• Hedgehog Pathway Research: Serves as a critical reagent for studying the inhibition of the Smoothened (SMO) receptor and downstream signaling events in real-time.
• Cancer Biology Studies: Applied in research targeting Hedgehog-dependent cancers, such as basal cell carcinoma and medulloblastoma, for mechanistic and efficacy studies.
• High-Content Screening (HCS): Utilized in automated imaging assays to identify and quantify cellular responses to Hedgehog pathway modulation.
• Flow Cytometry: Enables the analysis and sorting of cell populations based on cyclopamine binding or pathway activity status.
• Biochemical Assay Development: Acts as a tracer in competitive binding assays and other in vitro studies to characterize new inhibitors.

Basic Information

Product Name	Bodipy Cyclopamine
CAS No.	334658-24-1
Molecular Formula	C41H51BF2N2O3
Molecular Weight	656.67 g/mol
Synonyms	BODIPY-Cyclopamine; BODIPY® FL Cyclopamine; 11-Deoxojervine BODIPY conjugate; 3-[(3S,3aR,4S,5aR,6R,6aS,7S,9aR,9bS,10R)-4,6-Dihydroxy-3-(1-hydroxyethyl)-3a,5a,6a,7,9a,9b-hexahydro-3H-cyclopenta[a]phenanthren-10-yl]-N-[2-(5,5-difluoro-1,3,7,9-tetramethyl-5H-4l4,5l4-dipyrrolo[1,2-c:2',1'-f][1,3,2]diazaborinin-10-yl)ethyl]propanamide
EINECS	Contact for details

Quality Control

Our Bodipy Cyclopamine is produced under strict quality protocols to ensure high purity and batch-to-batch consistency for research reproducibility. Each lot is characterized by advanced analytical techniques, including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and fluorescence properties is provided with every shipment to support your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature before opening to minimize moisture condensation. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red solid or film
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~504 nm
Emission Maximum (λem)	~511 nm
Solubility	Soluble in DMSO, DMF, ethanol
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.