Description

5-TAMRA NHS Ester is a high-purity, amine-reactive fluorescent labeling reagent. This compound is critical for creating stable, covalent conjugates with primary amines in peptides, proteins, antibodies, and other biomolecules, enabling sensitive detection and analysis. It is an essential tool for researchers and manufacturers in life sciences, diagnostics, and biopharmaceutical development who require reliable, bright orange-red fluorescence for applications like fluorescence microscopy, flow cytometry, and immunoassays.

Application

• Biomolecule Labeling: Covalent conjugation to peptides, proteins, antibodies, and oligonucleotides for fluorescence-based assays.
• Proteomics & Cell Biology: Fluorescent tagging of cellular components for visualization via fluorescence microscopy (e.g., confocal, super-resolution).
• Diagnostic Assay Development: Used as a detection label in ELISA, lateral flow assays, and other immunoassay platforms.
• Flow Cytometry: Labeling of cell surface markers or intracellular proteins for cell sorting and analysis.
• Drug Discovery & Development: Tracking and localization studies of drug candidates and biomolecular interactions.
• Material Science: Functionalization of nanoparticles and polymers for bioimaging and sensing applications.

Basic Information

Product Name	5-TAMRA NHS Ester
CAS No.	321862-17-3
Molecular Formula	C26H21N3O7
Molecular Weight	487.46 g/mol
Synonyms	5-Carboxytetramethylrhodamine N-succinimidyl ester; 6-TAMRA, SE; 5-TAMRA, SE; Tetramethylrhodamine-5-isothiocyanate isomer; 5-Carboxy-tetramethylrhodamine N-hydroxysuccinimide ester; 5-TAMRA NHS; 5-TMR NHS ester; TAMRA-5-NHS
EINECS	Contact for details

Quality Control

Our 5-TAMRA NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥95%
Absorption Maximum (λmax)	~540-550 nm
Emission Maximum (λem)	~565-580 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.