Description

4-Hydroperoxy Cyclophosphamide is a key activated metabolite and synthetic precursor of the widely used chemotherapeutic agent cyclophosphamide. This compound matters significantly in pharmaceutical research and development as a critical reference standard and a direct-acting alkylating agent for mechanistic studies. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and contract research organization (CRO) sectors for drug metabolism investigations, impurity profiling, and the development of novel oncology therapies.

Application

• Pharmaceutical Reference Standard: Used as a high-purity certified standard for quality control (QC) and analytical method development in the production of cyclophosphamide and related prodrugs.
• Biomedical Research: Serves as a direct-acting alkylating agent in in vitro and ex vivo studies to investigate the mechanisms of action, cytotoxicity, and DNA cross-linking properties of activated cyclophosphamide without requiring metabolic activation.
• Metabolite Studies: Essential for pharmacokinetic and pharmacodynamic research, enabling the study of the biological activity and distribution of this primary active metabolite.
• Impurity Profiling: Employed in the identification and quantification of process-related impurities and degradation products in cyclophosphamide drug substance and finished dosage forms.
• Oncology Drug Development: Utilized as a biochemical tool in screening assays and the development of new chemotherapeutic regimens and combination therapies.
• Toxicology Assessment: Applied in studies to understand the specific toxicological profile and side effects associated with the activated form of the drug.

Basic Information

Item	Detail
Product Name	4-Hydroperoxy Cyclophosphamide
CAS No.	39800-16-3
Molecular Formula	C7H15Cl2N2O3P
Molecular Weight	277.09 g/mol
Synonyms	4-Hydroperoxycyclophosphamide; 4-HP-CPA; 4-Hydroperoxy-CPA; Peroxycyclophosphamide; Cyclophosphamide Monoperoxide; Cyclophosphamide 4-Hydroperoxide; NSC-181815; Aldophosphamide Peroxide
EINECS	Contact for details

Quality Control

Our 4-Hydroperoxy Cyclophosphamide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for pharmaceutical research and reference standard applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. Due to its hygroscopic and thermally sensitive nature, allow the sealed container to equilibrate to room temperature before opening to minimize moisture uptake and degradation. For long-term storage, keep desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	90.0% - 110.0%
Water Content (KF)	≤ 2.0%
Related Substances (HPLC)	Individual impurity ≤ 1.0%; Total impurities ≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.