Description

Hydroethidine is a vital fluorescent probe and chemical reagent widely used in biochemical and biomedical research. Its primary value lies in its specific reactivity with superoxide radicals, making it an essential tool for the detection and quantification of reactive oxygen species (ROS) in cellular systems. This compound is critically needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors for applications in oxidative stress studies, drug discovery, and diagnostic assay development.

Application

• Superoxide Radical Detection: Selective fluorescent detection of intracellular superoxide anion (O₂•⁻) in live cells and tissues.
• Oxidative Stress Research: Monitoring ROS production and oxidative damage in models of inflammation, neurodegeneration, and ischemia-reperfusion injury.
• Drug Efficacy Screening: Evaluating the antioxidant potential or pro-oxidant effects of novel pharmaceutical compounds in vitro.
• Mitochondrial Function Analysis: Assessing mitochondrial superoxide generation as an indicator of metabolic health and dysfunction.
• Diagnostic Assay Development: Serving as a key component in kits and assays for clinical and research-based measurement of oxidative stress biomarkers.
• Neuroscience Studies: Investigating the role of superoxide in neuronal signaling, aging, and neuroinflammatory pathways.

Basic Information

Product Name	Hydroethidine
CAS No.	38483-26-0
Molecular Formula	C₂₁H₂₁N₃
Molecular Weight	315.41 g/mol
Synonyms	Dihydroethidium; Hydroethidine; DHE; Reduced Ethidium; 5-Ethyl-5,6-dihydro-6-phenyl-3,8-diaminophenanthridine; HE; Ethidium homodimer-1 (reduced form); Superoxide Indicator
EINECS	Contact for details

Quality Control

Our Hydroethidine is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard assay systems. We adhere to high-purity reagent standards, ensuring the product meets the exacting requirements of analytical and life science research.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the product label or COA. For long-term storage, consider keeping the product desiccated at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Red to brown solid or powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Fluorescence Performance	Conforms to reference standard in superoxide detection assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.