Description

3-Nitrotetrazolium Blue Chloride is a vital tetrazolium salt derivative widely used as a redox indicator and chromogenic substrate in biochemical assays. Its primary value lies in its ability to form a highly colored, insoluble formazan dye upon reduction, making it indispensable for detecting cellular metabolic activity and enzymatic function. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, particularly for applications like cell viability testing (MTT assay alternatives) and dehydrogenase enzyme histochemistry.

Application

• Cell Viability and Proliferation Assays: Used as a substrate in colorimetric assays to measure mitochondrial activity in live cells, serving as an alternative to MTT.
• Dehydrogenase Enzyme Detection: Acts as an electron acceptor in histochemical staining to localize and quantify dehydrogenase enzyme activity in tissue sections.
• Microbiological Testing: Employed in diagnostic kits and methods to indicate bacterial growth and metabolic activity.
• Biochemical Research: Functions as a redox indicator in various experimental protocols studying electron transport chains and oxidative stress.
• Pharmaceutical Quality Control: Utilized in sterility testing and cytotoxicity assays during drug development and manufacturing.
• Plant Physiology Studies: Applied to assess seed viability and root dehydrogenase activity.

Basic Information

Product Name	3-Nitrotetrazolium Blue Chloride
CAS No.	38184-50-8
Molecular Formula	C40H30Cl2N10O6
Molecular Weight	817.64 g/mol
Synonyms	Nitrotetrazolium Blue Chloride; NTB Chloride; Tetrazolium Blue Chloride; 2,2'-Di-p-nitrophenyl-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylene)ditetrazolium Chloride; Blue Tetrazolium; BT; Nitro BT; 3,3'-(3,3'-Dimethoxy[1,1'-biphenyl]-4,4'-diyl)bis(2,5-diphenyl-2H-tetrazolium) dichloride
EINECS	Contact for details

Quality Control

Our 3-Nitrotetrazolium Blue Chloride is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including purity assay by HPLC, identity confirmation (IR, NMR), and checks for relevant impurities. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is kept sealed in a dry environment to prevent absorption of water vapor.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0 %
Residue on Ignition	≤ 0.5 %
Heavy Metals (as Pb)	≤ 20 ppm
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Biological Activity Test	Passes performance test in standard formazan generation assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.