Description

Dimidium Bromide Monohydrate is a vital cationic phenanthridine dye and nucleic acid stain used extensively in biological research and diagnostic applications. Its primary value lies in its ability to selectively bind to nucleic acids, making it an essential tool for visualizing DNA and RNA in various analytical techniques. This high-purity compound is critical for researchers and manufacturers in the biotechnology, pharmaceutical, and molecular diagnostics sectors who require reliable, consistent performance in sensitive assays.

Application

• Fluorescent Staining in Molecular Biology: Used as a fluorescent nucleic acid stain in gel electrophoresis, particularly for visualizing DNA fragments.
• Cell Viability Assays: Employed in combination with other dyes (e.g., ethidium bromide) for live/dead cell differentiation in microbiology and cell culture.
• Veterinary and Clinical Diagnostics: Acts as a key component in staining kits for the microscopic detection of blood parasites like Trypanosoma.
• Biochemical Research: Serves as a probe for studying nucleic acid structure, function, and protein interactions in vitro.
• Quality Control in Biotechnology: Utilized in processes to assess nucleic acid purity and concentration in sample preparation.

Basic Information

Product Name	Dimidium Bromide Monohydrate
CAS No.	37889-60-4
Molecular Formula	C20H18BrN3 · H2O
Molecular Weight	418.29 g/mol (Monohydrate)
Synonyms	3,8-Diamino-5-methyl-6-phenylphenanthridinium bromide monohydrate; Dimidium Bromide; Homidium Bromide; Ethidium Bromide Homologue; RD 1572; Trypan Red; Phenanthridinium, 3,8-diamino-5-methyl-6-phenyl-, bromide, monohydrate
EINECS	Contact for details

Quality Control

Our Dimidium Bromide Monohydrate is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure high purity and consistency, meeting the stringent requirements of research and diagnostic applications. A detailed Certificate of Analysis (COA) providing purity, identity, and impurity profiles is supplied with every shipment to guarantee traceability and compliance.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Reddish-brown to dark red crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0% (on anhydrous basis)
Water Content (KF)	3.5 - 5.0 %
Loss on Drying	≤ 5.5 %
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.1 %
Solubility	Soluble in water and ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.