Description

Rhodamine B Isothiocyanate is a reactive fluorescent dye derivative of Rhodamine B, widely used as a labeling reagent for proteins and other biomolecules. Its isothiocyanate group enables covalent conjugation to primary amines, creating stable fluorescent conjugates for sensitive detection. This product is essential for researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors who require high-performance fluorescent probes for applications such as flow cytometry, fluorescence microscopy, and immunoassay development.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amine groups (e.g., lysine residues) for creating fluorescent probes used in immunofluorescence (IF), immunohistochemistry (IHC), and Western blotting.
• Flow Cytometry and Cell Analysis: Preparation of fluorescently-tagged antibodies or other ligands for cell surface marker detection and intracellular staining.
• Fluorescence Microscopy and Imaging: Used to label biological specimens for high-resolution visualization of cellular structures and processes.
• Diagnostic Assay Development: Key component in the manufacture of fluorescent lateral flow assays, ELISA kits, and other in-vitro diagnostic (IVD) reagents.
• Bioconjugation and Probe Synthesis: A versatile building block for synthesizing custom fluorescent probes, sensors, and molecular beacons.
• Materials Science Research: Labeling of polymers, nanoparticles, or other materials to study their distribution, uptake, or degradation.

Basic Information

Product Name	Rhodamine B Isothiocyanate
CAS No.	36877-69-7
Molecular Formula	C29H29N3O3S
Molecular Weight	499.63 g/mol
Synonyms	Rhodamine B isothiocyanate; RBITC; Rhodamine B isothiocyanate isomer; [9-(2-Isothiocyanatophenyl)-6-diethylamino-3H-xanthen-3-ylidene]-diethylammonium chloride; Xanthylium, 9-[2-(isothiocyanato)phenyl]-3,6-bis(ethylamino)-, chloride; Rhodamine B isothiocyanate mixed isomers; Rhodamine B ITC; Basic Red 11 isothiocyanate
EINECS	253-242-3

Quality Control

Our Rhodamine B Isothiocyanate is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment and functional group verification, to ensure consistent performance for critical bioconjugation applications. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming key specifications such as identity, purity, and fluorescence properties.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions may affect its reactivity and fluorescence performance. For long-term stability, store desiccated under inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 90% (Mixed isomers)
Fluorescence Properties	Excitation/Emission: ~555/580 nm (Bound form)
Solubility	Soluble in DMF, DMSO, acetonitrile
Loss on Drying	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.