Description

Methyl Green-Pyronin CAS NO 36148-59-1 is a high-purity, dual-component biological stain composed of methyl green and pyronin Y. This specialized compound is critical for precise histological and cytological analysis, enabling clear differentiation of cellular structures. It is an essential reagent for research and diagnostic laboratories in the life sciences, biotechnology, and pharmaceutical development sectors.

Application

• Histological Staining: Primary application in the Methyl Green-Pyronin (MGP) stain for distinguishing DNA (green) and RNA (red) in tissue sections.
• Cytological Analysis: Used in blood smears and bone marrow aspirates to identify plasma cells and other RNA-rich cellular components.
• Microscopy & Pathology: A key diagnostic tool in research and clinical pathology for studying cell proliferation, apoptosis, and nucleic acid distribution.
• Botanical Studies: Employed in plant biology to stain chromosomes and study cell nuclei.
• Forensic Science: Utilized in certain forensic applications for biological specimen analysis.
• Biomedical Research: Essential for in-vitro studies requiring precise visualization of nucleic acids within cells.

Basic Information

Product Name	Methyl Green-Pyronin
CAS No.	36148-59-1
Molecular Formula	C41H42Cl2N8 • C17H19ClN2O
Molecular Weight	~ 958.28 g/mol (Methyl Green) + ~ 302.80 g/mol (Pyronin Y)
Synonyms	Methyl Green Pyronin Y; Methyl Green-Pyronin Y; MGP Stain; Methyl Green and Pyronin; Methyl Green Pyronin mixture; Methylgreen-Pyronin; Unna-Pappenheim stain component; Pyronin-Methyl Green.
EINECS	Contact for details

Quality Control

Our Methyl Green-Pyronin is manufactured under strict quality management systems. Each batch is subjected to rigorous analytical testing, including spectrophotometric analysis and chromatographic purity checks, to ensure consistent performance and meet the exacting standards required for biological staining. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and performance in staining is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Dark green crystalline powder or granules
Identification (IR)	Conforms to structure
Absorbance Spectrum	Conforms to reference (Methyl Green ~630nm, Pyronin ~550nm)
Staining Performance	Yields clear differentiation of DNA (green) and RNA (red) in control tissue sections
Solubility	Soluble in water, slightly soluble in ethanol
Loss on Drying	≤ 5.0 %
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.