Description

Propidium CAS NO 36015-30-2 is a vital fluorescent dye and nucleic acid stain widely used in cell biology and analytical chemistry. Its core value lies in its ability to selectively bind to DNA and RNA, making it an indispensable tool for cell viability assays, flow cytometry, and fluorescence microscopy. This high-purity compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors.

Application

• Cell Viability and Cytotoxicity Assays: Used as a membrane-impermeant dye to distinguish between live and dead cells by staining the DNA of cells with compromised membranes.
• Flow Cytometry Analysis: A critical reagent for DNA content and cell cycle analysis, providing quantitative data on cellular DNA.
• Fluorescence Microscopy: Employed as a nuclear counterstain to visualize cell nuclei in fixed or dead cell populations, often in conjunction with other fluorescent probes.
• Nucleic Acid Staining in Gel Electrophoresis: Used to stain DNA in agarose gels for visualization under UV light, though less common than ethidium bromide.
• Apoptosis Detection: Utilized in assays to identify cells in the late stages of apoptosis where membrane integrity is lost.
• Bacterial Viability Testing: Applied in microbiology to assess bacterial cell membrane integrity and enumerate viable versus non-viable cells.

Basic Information

Product Name	Propidium
CAS No.	36015-30-2
Molecular Formula	C27H34N4
Molecular Weight	414.59 g/mol
Synonyms	Propidium Iodide; 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide; Phenanthridinium, 3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenyl-, diiodide; PI; Propidium diiodide; Cell Viability Stain
EINECS	Contact for details

Quality Control

Our Propidium is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, fluorescence properties, and absence of critical impurities is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Dark red to reddish-brown powder or crystals
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (Analytical Grade)
Absorbance (Max)	~535 nm in aqueous solution
Fluorescence (Emission Max)	~617 nm (with DNA binding)
Loss on Drying	≤ 2.0 %
Solubility	Soluble in water and DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.