Description

Resorufin Sodium Salt CAS NO 34994-50-8 is a high-purity, water-soluble derivative of resorufin, a versatile and highly fluorescent xanthene dye. This compound is a critical reagent in enzymatic and cellular assays, serving as a sensitive fluorescent probe and substrate. It is primarily utilized by research institutions, diagnostic manufacturers, and pharmaceutical companies engaged in life science research, high-throughput screening, and diagnostic development.

Application

• Enzymatic Assay Substrate: Widely used as a fluorogenic substrate for detecting and quantifying enzyme activity, particularly for oxidoreductases like cytochrome P450 and peroxidases.
• Cell Viability & Cytotoxicity Testing: Employed in assays such as the resazurin reduction test (Alamar Blue) to measure metabolic activity and cell proliferation.
• Biomarker Detection: Acts as a fluorescent tag or reporter in various immunoassays and histochemical staining procedures.
• Reactive Oxygen Species (ROS) Detection: Used in probes to detect and quantify intracellular oxidative stress and hydrogen peroxide production.
• High-Throughput Screening (HTS): A key component in fluorescent assay kits for drug discovery and pharmacological profiling in automated screening systems.
• Diagnostic Kit Manufacturing: Incorporated as a core reagent in the production of clinical diagnostic kits for various enzymatic analyses.
• Research Chemical: Serves as a fundamental tool in biochemical and molecular biology research for developing new analytical methods.

Basic Information

Product Name	Resorufin Sodium Salt
CAS No.	34994-50-8
Molecular Formula	C12H6NNaO4
Molecular Weight	251.17 g/mol
Synonyms	7-Hydroxy-3H-phenoxazin-3-one sodium salt; Resazurin Sodium Salt (reduced form); Sodium Resorufin; 3H-Phenoxazin-3-one, 7-hydroxy-, sodium salt (1:1); 7-Hydroxyphenoxazin-3-one sodium salt; Amplex Red (reduced product); AAT Bioquest Cat. No. 17550
EINECS	Contact for details

Quality Control

Our Resorufin Sodium Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, spectroscopy for identity confirmation, and residual solvent analysis, to ensure it meets the stringent requirements for research and diagnostic applications. Certificates of Analysis (COA) with detailed lot-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive (store away from light). For long-term stability, consider storage at 2-8°C under desiccated conditions.

Specification

Item	Specification
Appearance	Dark red to brownish-red powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Fluorescence	Characteristic emission spectrum
Solubility	Soluble in water, DMSO, and methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.