Description

Bolton-Hunter Reagent CAS NO 34071-95-9 is a specialized, activated ester derivative of N-succinimidyl 3-(4-hydroxyphenyl)propionate, widely used as a critical labeling agent for proteins and peptides. Its primary value lies in enabling the efficient radioiodination of biomolecules for sensitive detection and quantification in research and diagnostics. This reagent is essential for scientists and manufacturers in the fields of radiopharmaceutical development, immunoassay production, and biomedical research, particularly for creating tracers used in imaging and binding studies.

Application

• Radioiodination of Proteins and Peptides: Primary application for labeling tyrosine residues with iodine-125 or iodine-131.
• Tracer Synthesis for Radioimmunoassays (RIA): Production of high-specific-activity radiolabeled antigens and antibodies.
• In Vitro Receptor Binding Studies: Creation of labeled ligands to study receptor density, affinity, and pharmacology.
• Metabolic Pathway Tracing: Labeling of biomolecules to track uptake, distribution, and catabolism in biological systems.
• Diagnostic Imaging Agent Development: A key precursor in the synthesis of radiopharmaceuticals for SPECT imaging.
• Quality Control in Biopharmaceuticals: Used in internalization assays and stability studies of therapeutic proteins.

Basic Information

Product Name	Bolton-Hunter Reagent
CAS No.	34071-95-9
Molecular Formula	C14H13NO5
Molecular Weight	275.26 g/mol
Synonyms	N-Succinimidyl 3-(4-Hydroxyphenyl)propionate; SHPP; Bolton and Hunter Reagent; 3-(4-Hydroxyphenyl)propionic Acid N-Hydroxysuccinimide Ester; N-Hydroxysuccinimide Ester of 3-(p-Hydroxyphenyl)propionic Acid; 3-(p-Hydroxyphenyl)propionic Acid N-Succinimidyl Ester; NHS Ester of 3-(4-Hydroxyphenyl)propionic Acid
EINECS	Contact for details

Quality Control

Our Bolton-Hunter Reagent is manufactured under strict quality protocols to ensure high purity and consistent performance for sensitive applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing key parameters such as assay purity, impurity profile, and functional suitability. We adhere to cGMP principles where applicable, ensuring traceability and reliability for research and development use.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	155 - 160 °C
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMF, DMSO, acetonitrile
Functional Suitability	Conforms (Iodination Test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.