Description

o-Tolyltetrazolium Red is a specialized tetrazolium salt derivative, widely recognized as a vital redox indicator and biochemical reagent. Its primary value lies in its ability to serve as a sensitive chromogenic substrate for detecting cellular metabolic activity, particularly in assays for cell viability, cytotoxicity, and dehydrogenase enzymes. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise measurement of biological activity is critical.

Application

• Cell Viability and Proliferation Assays: Used as a key substrate in MTT and related assays to quantify metabolically active cells.
• Microbiological Testing: Employed as an indicator in tests for bacterial contamination and antimicrobial susceptibility.
• Enzyme Activity Studies: Acts as an electron acceptor in assays for dehydrogenase and reductase enzymes.
• Histochemical Staining: Utilized in tissue sections to localize sites of metabolic activity.
• Pharmaceutical Research: Applied in high-throughput screening (HTS) for drug discovery and toxicology studies.
• Food and Beverage Analysis: Used in rapid microbiological methods for quality control.
• Diagnostic Kit Manufacturing: Incorporated as a core component in commercial diagnostic test kits.

Basic Information

Product Name	o-Tolyltetrazolium Red
CAS No.	33926-00-0
Molecular Formula	C₁₉H₁₉N₅O
Molecular Weight	333.39 g/mol
Synonyms	2-(2-Methoxyphenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride; o-Methoxyphenyltetrazolium Red; MTT Red; Tetrazolium Red o-tolyl; o-Tolyl Red Tetrazolium; 2-(o-Methoxyphenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride; INT (related tetrazolium salt class)
EINECS	Contact for details

Quality Control

Our o-Tolyltetrazolium Red is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including purity assay by HPLC, identity confirmation (IR, NMR), and tests for residual solvents and moisture. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to maintain stability and performance.

Specification

Item	Specification
Appearance	Dark red to reddish-brown powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, methanol; sparingly soluble in water
Biological Activity	Meets performance criteria in standard cell viability assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.