Description

Bromophenol Blue Sodium Salt is a high-purity, water-soluble derivative of the widely used pH indicator bromophenol blue. This compound is essential for precise colorimetric analysis and endpoint detection in a variety of laboratory and industrial processes. It is primarily utilized by professionals in life sciences, analytical chemistry, and quality control laboratories. Its consistent performance makes it a critical reagent for applications requiring reliable and sensitive pH indication.

Application

• pH Indicator in Electrophoresis: A standard tracking dye in agarose and polyacrylamide gel electrophoresis (PAGE) for monitoring the progress of nucleic acid and protein separation.
• Endpoint Detection in Titrations: Used as a visual indicator in acid-base titrations within its effective pH transition range (yellow to blue, ~3.0-4.6).
• Microbiological Media: Incorporated into selective culture media as a pH indicator to differentiate microbial growth based on metabolic acid production.
• Biochemical Staining: Employed in histological and cytological staining protocols to provide contrast and highlight specific tissue structures.
• Analytical Chemistry Standards: Serves as a reference standard and calibrant in spectroscopic and chromatographic methods for quality assurance.
• Educational & Research Laboratories: A fundamental reagent for teaching principles of acid-base chemistry and for various research applications in biochemistry.

Basic Information

Product Name	Bromophenol Blue Sodium Salt
CAS No.	33551-92-7
Molecular Formula	C19H9Br4O5S·Na
Molecular Weight	691.94 g/mol
Synonyms	3',3'',5',5''-Tetrabromophenolsulfonephthalein Sodium Salt; Bromphenol Blue Sodium Salt; BPB Sodium Salt; Tetrabromophenol Blue Sodium Salt; Acid Blue 90; C.I. 45405; Sodium 3',3'',5',5''-Tetrabromophenolsulfonephthalein; 4,4'-(1,1-Dioxido-3H-2,1-benzoxathiole-3,3-diyl)bis(2,6-dibromophenol) Sodium Salt
EINECS	251-560-9

Quality Control

Our Bromophenol Blue Sodium Salt is manufactured under a strict quality management system. Every batch undergoes comprehensive analytical testing to ensure it meets high-purity standards suitable for critical laboratory use. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and conformance to specifications. We support compliance with ISO and GMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Dark blue to purple powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 95.0%
pH Transition Range	3.0 (yellow) to 4.6 (blue)
Loss on Drying	≤ 5.0 %
Solubility (1% in Water)	Clear, blue solution
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.