Description

4-Nitrophenyl Iodoacetate is a specialized organic reagent featuring a reactive iodoacetate ester group. This compound matters as a versatile building block and labeling agent, particularly valuable for the selective modification of thiol groups in peptides, proteins, and other biomolecules. It is primarily needed by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for applications in proteomics, drug discovery, and biochemical assay development.

Application

• Bioconjugation & Protein Labeling: Acts as a thiol-specific alkylating agent for covalent modification of cysteine residues in proteins and peptides.
• Proteomics Research: Used in activity-based protein profiling (ABPP) and chemoproteomic studies to identify and characterize enzyme active sites.
• Chemical Biology Probe Synthesis: Serves as a key intermediate in the synthesis of activity-based probes (ABPs) for studying enzymatic function.
• Pharmaceutical Intermediate: Employed in the research and development of targeted therapeutics and diagnostic agents.
• Biochemical Assay Development: Utilized in the design of colorimetric or fluorogenic substrates for enzyme activity detection.
• Organic Synthesis: Functions as an acylating or alkylating agent in the preparation of more complex organic molecules.

Basic Information

Product Name	4-Nitrophenyl Iodoacetate
CAS No.	31252-85-4
Molecular Formula	C8H6INO4
Molecular Weight	307.05 g/mol
Synonyms	p-Nitrophenyl Iodoacetate; Iodoacetic Acid 4-Nitrophenyl Ester; 4-Nitrophenyl 2-Iodoacetate; (4-Nitrophenoxy)carbonylmethyl Iodide; 2-Iodoacetic Acid 4-Nitrophenyl Ester; Iodoacetic Acid p-Nitrophenyl Ester; PNIA; 4-Nitrophenoxycarbonylmethyl Iodide
EINECS	Contact for details

Quality Control

Our 4-Nitrophenyl Iodoacetate is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C to -10°C under an inert atmosphere (e.g., nitrogen or argon) to minimize oxidation and degradation. Allow the container to reach room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.