Description

Ethidium is a vital cationic phenanthridine dye widely recognized for its strong fluorescence upon intercalation with nucleic acids. This property makes it an indispensable tool for visualizing and quantifying DNA and RNA in molecular biology and biochemistry. It is a critical reagent for researchers and industrial laboratories engaged in genetic analysis, virology, and pharmaceutical development. Ethidium CAS NO 3546-21-2 is supplied to meet the stringent purity requirements of advanced life science applications.

Application

• Gel Electrophoresis Staining: Primary application for visualizing DNA/RNA bands in agarose and polyacrylamide gels.
• Fluorescent Probe in Cytometry: Used in flow cytometry for cell cycle analysis and apoptosis detection by staining nuclear DNA.
• Nucleic Acid Quantification: Employed in fluorometric assays to determine the concentration of DNA and RNA samples.
• Virology Research: Used to stain and study viral plaques and to investigate virus-host cell interactions.
• Mitochondrial Studies: Acts as a probe for mitochondrial DNA due to its selective accumulation in live cells.
• Antitrypanosomal Agent: Serves as a starting material or reference standard in the development of veterinary trypanocides.
• Laboratory Reagent: A key component in various molecular biology kits and buffer formulations.

Basic Information

Product Name	Ethidium
CAS No.	3546-21-2
Molecular Formula	C21H20N3+
Molecular Weight	314.40 g/mol
Synonyms	Ethidium Bromide; Homidium Bromide; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium Bromide; 2,7-Diamino-10-ethyl-9-phenylphenanthridinium Bromide; Novidium Bromide; RD 1572; EBr; EtBr
EINECS	222-581-2

Quality Control

Our Ethidium is manufactured under a strict quality management system. Every batch undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance profile verification, and loss on drying. A Certificate of Analysis (COA) detailing all test results is provided to ensure traceability and compliance with your research or production specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Dark red to brown crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 95.0%
Absorbance Ratio (A280/A260)	≤ 0.30 (in H2O)
Loss on Drying	≤ 5.0 %
Fluorescence	Suitable for nucleic acid staining

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.