Description

6-Carboxyfluorescein Diacetate is a cell-permeable, non-fluorescent ester derivative of fluorescein, widely used as a vital intracellular probe. Upon entering living cells, endogenous esterases cleave the acetate groups, releasing the highly fluorescent 6-carboxyfluorescein, enabling real-time monitoring of cell viability, membrane integrity, and enzyme activity. This compound is essential for researchers and quality control professionals in the biotechnology, pharmaceutical, and diagnostic industries for applications ranging from cytotoxicity assays to cell tracing.

Application

• Cell Viability and Cytotoxicity Assays: Used as a vital stain to distinguish between live and dead cells based on intracellular esterase activity.
• Cell Tracing and Proliferation Studies: Functions as a long-term cell tracer for monitoring cell migration, division, and population dynamics in vitro.
• Enzyme Activity Detection: Serves as a substrate for detecting and quantifying esterase enzyme activity in biochemical and pharmacological research.
• Membrane Integrity and Permeability Tests: Employed to assess plasma membrane integrity, as fluorescence is retained only in cells with intact membranes.
• Flow Cytometry and Fluorescence Microscopy: A key fluorescent probe for quantitative analysis and imaging of cellular processes.
• Drug Screening: Utilized in high-throughput screening platforms to evaluate the effects of drug candidates on cell health and enzymatic function.

Basic Information

Product Name	6-Carboxyfluorescein Diacetate
CAS No.	3348-03-6
Molecular Formula	C25H18O9
Molecular Weight	462.41 g/mol
Synonyms	6-CFDA; 6-Carboxyfluorescein Diacetate, Succinimidyl Ester (precursor); 5(6)-Carboxyfluorescein Diacetate; CFDA; 3',6'-Diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic Acid; Fluorescein Diacetate, 6-Carboxy-; CFDA, SE (for activated ester); 6-Carboxyfluorescein Diacetate, Mixed Isomers
EINECS	Contact for details

Quality Control

Our 6-Carboxyfluorescein Diacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biological applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/IR)	Conforms to standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.