Description

5-Aminofluorescein is a vital fluorescent dye derivative and a key synthetic intermediate in the production of advanced diagnostic reagents. Its primary value lies in its reactive amino group, which enables facile conjugation to biomolecules like antibodies, proteins, and oligonucleotides for labeling applications. This compound is essential for researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors who require high-purity fluorescent probes.

Application

• Fluorescent Labeling Reagent: Used as a precursor for synthesizing amine-reactive fluorescent probes (e.g., FITC derivatives) for labeling antibodies, proteins, and nucleic acids.
• Bioconjugation & Probe Development: Serves as a critical intermediate in the creation of custom fluorescent conjugates for flow cytometry, immunofluorescence (IF), and fluorescence microscopy.
• Diagnostic Assay Manufacturing: Employed in the production of fluorescent detection kits for clinical diagnostics, ELISA, and in-situ hybridization (FISH).
• Biomedical Research: Utilized as a tracer or tag in cellular imaging, receptor binding studies, and tracking biological pathways.
• Sensor & Detector Chemistry: Acts as a building block for developing fluorescent chemical sensors and indicators for pH, metal ions, or enzymatic activity.
• Material Science: Can be incorporated into polymers or nanoparticles to create fluorescent materials for security inks or advanced coatings.

Basic Information

Product Name	5-Aminofluorescein
CAS No.	3326-34-9
Molecular Formula	C20H13NO5
Molecular Weight	347.32 g/mol
Synonyms	5-Aminofluorescein; 3',6'-Dihydroxy-5-amino-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 5-AF; Fluoresceinamine; 5-Amino-3',6'-dihydroxyfluoran; 5-Amino-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 5-Amino-3',6'-dihydroxyfluorane; Aminofluorescein
EINECS	222-045-2

Quality Control

Our 5-Aminofluorescein is produced under strict quality management systems to ensure batch-to-batch consistency and high purity, critical for sensitive bioconjugation work. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment. We support compliance with relevant industry standards for research and in-vitro diagnostic (IVD) manufacturing.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. Keep the container tightly sealed when not in use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	Orange to red-brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Heavy Metals (as Pb)	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.