Description

4-Aminofluorescein is a vital fluorescent dye derivative, widely recognized for its role as a key intermediate in synthesizing advanced labeling reagents. Its primary value lies in its ability to be readily conjugated to biomolecules, enabling sensitive detection and imaging applications across various scientific fields. This compound is essential for researchers and manufacturers in the life sciences, diagnostics, and analytical chemistry sectors who require high-purity fluorescent tags and probes.

Application

• Fluorescent Labeling Reagent Synthesis: A crucial precursor for creating amine-reactive fluorescent probes and tags (e.g., FITC derivatives) for protein and antibody conjugation.
• Biomolecular Imaging & Detection: Used in the development of fluorescent sensors and stains for cellular imaging, flow cytometry, and immunohistochemistry.
• Diagnostic Assay Development: Serves as a building block for fluorescent markers in ELISA, lateral flow assays, and other in vitro diagnostic (IVD) kits.
• Research & Development: Employed in academic and industrial R&D for studying molecular interactions, enzyme activity, and cellular processes via fluorescence.
• Material Science: Can be incorporated into polymers or nanoparticles to create functional fluorescent materials for sensing applications.

Basic Information

Product Name	4-Aminofluorescein
CAS No.	3326-33-8
Molecular Formula	C20H13NO5
Molecular Weight	347.32 g/mol
Synonyms	5-Aminofluorescein; 3',6'-Dihydroxy-4-amino-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one; 4-Amino-3',6'-dihydroxyfluoran; 4-AF; Fluoresceinamine; 4-Aminofluoran
EINECS	222-045-1

Quality Control

Our 4-Aminofluorescein is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity suitable for sensitive research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	Orange to red-brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.