Description

6-Carboxyfluorescein is a high-purity, fluorescent tracer dye widely used in biochemical and diagnostic applications. Its core value lies in its excellent water solubility and strong fluorescence emission, making it an indispensable tool for labeling and detection. This compound is essential for researchers and manufacturers in the life sciences, pharmaceutical development, and diagnostic kit production. It serves as a critical intermediate for synthesizing more complex fluorescent probes and conjugates.

Application

• Fluorescent Labeling Agent: Used to label proteins, antibodies, and nucleic acids for detection in assays like ELISA and Western blotting.
• Cell Biology Research: Employed as a tracer dye in cell permeability studies, vesicle labeling, and fluorescence microscopy.
• Diagnostic Reagent Intermediate: A key starting material for synthesizing fluorescent probes and conjugates used in clinical diagnostic kits.
• Capillary Electrophoresis (CE): Acts as a standard or marker in CE and other separation techniques due to its consistent fluorescent properties.
• Enzyme Substrate: Utilized in enzyme activity assays where enzymatic cleavage releases the fluorescent moiety.
• pH-Sensitive Fluorescent Indicator: Its fluorescence intensity is pH-dependent, making it useful in pH-sensing applications within physiological ranges.

Basic Information

Product Name	6-Carboxyfluorescein
CAS No.	3301-79-9
Molecular Formula	C21H12O7
Molecular Weight	376.32 g/mol
Synonyms	6-FAM; 6-Carboxyfluorescein, mixed isomers; Fluorescein 6-carboxylic acid; 3',6'-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-6-carboxylic acid; 6-CF; 6-Carboxy-3',6'-dihydroxyfluoran; 6-Carboxy-fluorescein; 6-Fluorescein carboxylic acid
EINECS	221-975-1

Quality Control

Our 6-Carboxyfluorescein is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment, ensuring traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0 %
Fluorescence	Conforms
Solubility	Soluble in DMSO, aqueous base

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.