Description

2-Nitrophenyl 2,3,4,6-Tetra-O-acetyl-β-D-galactopyranoside is a specialized glycoside derivative widely used as a chromogenic substrate in enzymatic assays. This compound is critical for research and development in biochemistry and molecular biology, particularly for the detection and quantification of β-galactosidase activity. It serves as an essential reagent for scientists in the pharmaceutical, diagnostic, and life science industries who require high-purity, reliable substrates for their analytical workflows.

Application

• Enzymatic Substrate for β-Galactosidase: Primary use as a chromogenic substrate in colorimetric assays to detect and measure enzyme activity.
• Biochemical Research: A key tool in molecular biology for studying gene expression, particularly in reporter gene systems like the lacZ operon.
• Diagnostic Kit Manufacturing: Used in the formulation of diagnostic kits for clinical and research laboratories to identify bacterial species or genetic markers.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) assays during drug discovery to identify modulators of enzymatic pathways.
• Quality Control in Biotechnology: Acts as a standard reagent for validating the performance and specificity of β-galactosidase enzymes used in industrial processes.
• Academic Research: Utilized in university and institutional labs for fundamental research in enzymology, microbiology, and cellular biology.

Basic Information

Product Name	2-Nitrophenyl 2,3,4,6-Tetra-O-acetyl-β-D-galactopyranoside
CAS No.	3053-17-6
Molecular Formula	C20H23NO12
Molecular Weight	469.40 g/mol
Synonyms	4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside; p-Nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside; PNPTAG; ONPGal tetraacetate; β-D-Galactopyranoside, 2-nitrophenyl 2,3,4,6-tetraacetate; 2-Nitrophenyl tetra-O-acetyl-β-D-galactopyranoside; Galactose tetraacetate 2-nitrophenyl glycoside
EINECS	Contact for details

Quality Control

Our 2-Nitrophenyl 2,3,4,6-Tetra-O-acetyl-β-D-galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive analytical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with relevant research and development standards, and specifications can be tailored to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Melting Point	158 - 162 °C
Specific Rotation	Contact for details
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.