Description

D-Ala-Lys-Amca (Hydrochloride) is a synthetic, fluorophore-labeled dipeptide derivative used as a critical biochemical tool in advanced research. Its value lies in its role as a highly specific and sensitive substrate for studying protease activity and enzyme kinetics. This compound is essential for researchers and developers in pharmaceutical discovery, proteomics, and diagnostic assay development, where precise measurement of enzymatic processes is required.

Application

• Protease Activity Assays: Used as a fluorogenic substrate to measure the activity of specific proteases, including trypsin-like enzymes.
• High-Throughput Screening (HTS): Employed in drug discovery campaigns to identify and characterize novel protease inhibitors.
• Enzyme Kinetic Studies: Facilitates the determination of kinetic parameters (Km, Vmax) for target enzymes.
• Biomarker Research: Applied in the development of diagnostic assays for detecting protease-related disease biomarkers.
• Cell-Based Imaging: Utilized in live-cell imaging studies to visualize and localize protease activity within biological systems.
• Biochemical Pathway Analysis: Aids in elucidating the role of specific proteolytic events in cellular signaling pathways.

Basic Information

Product Name	D-Ala-Lys-Amca (Hydrochloride)
CAS No.	2703746-41-0
Molecular Formula	C20H29ClN4O4
Molecular Weight	424.92 g/mol
Synonyms	D-Alanine-L-Lysine-7-Amino-4-methylcoumarin Hydrochloride; D-Ala-Lys-AMC HCl; D-Ala-Lys-AMCA HCl; (2R)-2-[[(2S)-6-amino-2-[[2-(4-methyl-2-oxochromen-7-yl)amino]-2-oxoethyl]amino]hexanoyl]amino]propanoic acid hydrochloride; AMC-labeled D-Ala-Lys dipeptide; Fluorogenic substrate D-Ala-Lys-AMC; Protease substrate 2703746-41-0
EINECS	Contact for details

Quality Control

Our D-Ala-Lys-Amca (Hydrochloride) is manufactured under strict quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance data. We adhere to rigorous analytical standards, ensuring the product meets the exacting requirements of life science research and development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conforms to reference standard in fluorogenic assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.