Description

Thalidomide-o-Peg4-Nhs Ester is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation and proteolysis-targeting chimera (PROTAC) linker applications. This compound matters for its precise ability to incorporate the thalidomide moiety, a well-known cereblon (CRBN) E3 ligase ligand, into complex molecules for targeted protein degradation research. Pharmaceutical researchers and biotech developers in the fields of oncology, immunology, and chemical biology need this specialized building block to create next-generation therapeutic candidates and sophisticated molecular probes.

Application

• PROTAC Linker Synthesis: A critical component for constructing Proteolysis-Targeting Chimeras, linking the thalidomide-based E3 ligase ligand to a target protein-binding warhead.
• Bioconjugation Reagent: Used to functionalize proteins, antibodies, or peptides with the thalidomide moiety via its amine-reactive NHS ester group, enabling the study of protein-protein interactions and degradation.
• Chemical Biology Probe Development: Facilitates the creation of molecular probes for investigating cereblon (CRBN) biology, substrate recruitment, and mechanisms of targeted protein degradation.
• ADC (Antibody-Drug Conjugate) Payload-Linker: Serves as a sophisticated linker component in the development of novel ADCs, particularly those employing degradation-based mechanisms of action.
• Small Molecule Drug Discovery: Acts as a key intermediate in medicinal chemistry programs focused on immunomodulatory imide drug (IMiD) analogs and targeted degradation platforms.
• Polyethylene Glycol (PEG) Spacer Integration: The integrated tetraethylene glycol (PEG4) spacer provides solubility and favorable pharmacokinetic properties to the final conjugate.

Basic Information

Product Name	Thalidomide-o-Peg4-Nhs Ester
CAS No.	2411681-88-2
Molecular Formula	C31H38N4O12
Molecular Weight	658.66 g/mol
Synonyms	Thalidomide-PEG4-NHS Ester; Thalidomide-O-PEG4-NHS; 2,5-Dioxopyrrolidin-1-yl 3-(2-(2-(2-(2-(2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindolin-4-yl)oxy)ethoxy)ethoxy)ethoxy)propanoate; Thalidomide-PEG4-OSu; CRBN Ligand-PEG4-NHS Ester; N-(2-(2-(2-(2-(3-(2,5-Dioxo-2,5-dihydro-1H-pyrrol-1-yl)propanoyloxy)ethoxy)ethoxy)ethoxy)ethyl)-2-(2,6-dioxopiperidin-3-yl)-1,3-dioxoisoindoline-5-carboxamide (alternative representation); Thalidomide tetraethyleneglycol NHS ester
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research and development. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and stability. Certificates of Analysis (COA) detailing lot-specific results are available upon request, supporting your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.