Description

Nh2-Peg-Fitc CAS NO 2411381-38-7 is a high-purity, functionalized polyethylene glycol (PEG) derivative featuring a primary amine (NH2) group for conjugation and a fluorescein isothiocyanate (FITC) moiety for fluorescent labeling. This bifunctional reagent is engineered to enable the precise, covalent attachment of biomolecules and polymers to surfaces or other molecules while providing a powerful fluorescent tag for detection and imaging. It is an essential tool for researchers and developers in the fields of bioconjugation, diagnostics, and advanced material science, where reliable, high-quality linkers are critical for success.

Application

• Bioconjugation and Protein Labeling: Covalently link the amine-reactive FITC group to proteins, antibodies, or peptides for fluorescence-activated cell sorting (FACS), immunofluorescence, and microscopy.
• Polymer Functionalization and Surface Modification: Utilize the terminal amine group to graft PEG chains onto nanoparticles, quantum dots, or solid surfaces to create stealth coatings, improve biocompatibility, and introduce reactive sites for further chemistry.
• Diagnostic Assay Development: Serve as a key component in the synthesis of fluorescent probes and biosensors for in vitro diagnostic (IVD) applications.
• Drug Delivery System Engineering: Act as a versatile linker in the construction of targeted drug delivery systems, such as antibody-drug conjugates (ADCs) or PEGylated liposomes, facilitating both attachment and tracking.
• Biomaterial and Hydrogel Synthesis: Incorporate into the design of smart hydrogels and biomaterials where fluorescent tracking of material degradation or cell interaction is required.
• Flow Cytometry and Cell Analysis: Label cell surface markers or intracellular targets for quantitative analysis and sorting in life science research.

Basic Information

Product Name	Nh2-Peg-Fitc
CAS No.	2411381-38-7
Molecular Formula	Contact for details
Molecular Weight	Variable (depends on PEG chain length)
Synonyms	Amino-PEG-Fluorescein Isothiocyanate; Amine-PEG-FITC; NH2-Polyethylene Glycol-FITC; α-Amino-ω-FITC Poly(ethylene glycol); FITC-PEG-Amine; Fluorescein Isothiocyanate-PEG-NH2; PEG-FITC-NH2; 5-Isothiocyanatofluorescein-PEG-amine
EINECS	Contact for details

Quality Control

Every batch of Nh2-Peg-Fitc is manufactured under strict quality management protocols. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic techniques, to ensure high purity, consistent performance, and compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, substitution ratio, and functional group integrity is provided with each shipment and is available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to yellow solid or viscous liquid
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Amine Substitution	≥ 90%
FITC Substitution	≥ 90%
Moisture Content (KF)	≤ 1.0%
PEG Average MW	As ordered (e.g., 2kDa, 5kDa)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.