Description

Cyanine7 Nhs Ester CAS NO 2408482-09-5 is a near-infrared (NIR) fluorescent labeling reagent, specifically a reactive succinimidyl ester derivative of the Cyanine7 dye. This compound is critically important for the covalent conjugation of the bright, stable Cyanine7 fluorophore to primary amines on proteins, peptides, antibodies, and other biomolecules. It is an essential tool for researchers and developers in bioconjugation, in vivo imaging, diagnostics, and pharmaceutical development, enabling the creation of highly specific fluorescent probes and targeted imaging agents.

Application

• Antibody-Dye Conjugate Preparation: Labeling monoclonal antibodies for fluorescence-activated cell sorting (FACS), immunofluorescence, and in vivo tumor imaging.
• Peptide & Protein Labeling: Conjugation to peptides, enzymes, and other proteins for tracking, binding studies, and molecular interaction analysis.
• Nanoparticle & Liposome Functionalization: Attaching NIR fluorescent tags to drug delivery carriers for real-time biodistribution and pharmacokinetic studies.
• In Vivo Imaging Probes: Synthesis of targeted optical imaging agents for preclinical research in oncology, neurology, and cardiology.
• Diagnostic Assay Development: Used as a key component in developing fluorescent immunoassays and lateral flow tests.
• Biomolecule Crosslinking: Acting as a homobifunctional crosslinker for amine-amine conjugation in probe construction.

Basic Information

Product Name	Cyanine7 NHS Ester
CAS No.	2408482-09-5
Molecular Formula	C41H45KN2O6S2
Molecular Weight	765.03 g/mol
Synonyms	Cyanine7 succinimidyl ester; Cy7 NHS ester; Sulfo-Cyanine7 NHS ester (potassium salt); 1-(6-((E)-2-((E)-2-(1-(5-Carboxypentyl)-3,3-dimethylindolin-2-ylidene)ethylidene)-5-((E)-2-(3,3-dimethyl-1-(5-(2,5-dioxopyrrolidin-1-yl)oxy)-5-oxopentyl)indolin-2-ylidene)ethylidene)-4-methylcyclohex-1-en-1-yl)vinyl)-3,3-dimethyl-1-(5-(2,5-dioxopyrrolidin-1-yl)oxy)-5-oxopentyl)indol-1-ium; NIR-783 NHS ester; Cy7® dye NHS ester
EINECS	Contact for details

Quality Control

Our Cyanine7 NHS Ester is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional performance is provided with every shipment, supporting compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under an inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	≥ 200,000 cm-1M-1 at ~750 nm
Water Content (KF)	≤ 2.0%
Single Spot (TLC)	Conforms
Functional Performance	Conjugation efficiency ≥ 90% (by control test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.