Description

Sulfo-Cyanine5.5 Azide is a near-infrared (NIR) fluorescent dye functionalized with a reactive azide group and water-solubilizing sulfonate moieties. This compound is engineered for high-performance bio-conjugation via copper-free click chemistry, enabling the labeling of biomolecules without compromising their native function or solubility. It is an essential tool for researchers and developers in biopharmaceuticals, diagnostics, and advanced materials science who require stable, bright, and biocompatible NIR probes for in vivo imaging, flow cytometry, and biomaterial tracking.

Application

• Bioconjugation & Probe Development: Site-specific labeling of antibodies, proteins, peptides, and oligonucleotides for imaging and detection.
• In Vivo Imaging: Labeling of nanoparticles, targeting agents, and cells for deep-tissue fluorescence imaging in preclinical studies.
• Diagnostic Assays: Development of fluorescent lateral flow assays (LFAs), ELISA, and other diagnostic platforms utilizing NIR detection.
• Flow Cytometry & Microscopy: Creation of bright, photostable fluorescent conjugates for cell sorting and high-resolution cellular imaging.
• Materials Science: Functionalization of polymers, hydrogels, and surfaces for biosensing and smart material applications.
• Chemical Biology: As a tool for activity-based protein profiling (ABPP) and tracking biomolecular interactions in complex systems.

Basic Information

Product Name	Sulfo-Cyanine5.5 Azide
CAS No.	2382994-65-0
Molecular Formula	C44H47KN4O9S2 (Potassium salt form typical)
Molecular Weight	~903.1 g/mol (Potassium salt)
Synonyms	Sulfo-Cy5.5 Azide; Cyanine5.5 sulfonyl azide; Sulfo-Cyanine 5.5 N3; Sulfo-Cy5.5 NHS ester analog (azide); Water-soluble Cy5.5 azide; NIR dye azide; 2-((1E,3E)-5-(1-(5-Azidopentyl)-3,3-dimethyl-5-sulfo-1,3-dihydro-2H-indol-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate; Potassium 2-((1E,3E)-5-(1-(5-azidopentyl)-3,3-dimethyl-5-sulfonato-1,3-dihydro-2H-indol-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate
EINECS	Contact for details

Quality Control

Our Sulfo-Cyanine5.5 Azide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical characterization, including HPLC for purity, mass spectrometry (MS) for identity confirmation, and spectrophotometry for absorbance/fluorescence validation. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Custom synthesis and purification to meet specific application requirements are available.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~680 - 690 nm
Emission Maximum (λem)	~700 - 710 nm
Extinction Coefficient (ε)	≥ 190,000 M-1cm-1
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.