Description

Azido-Peg24-Nhs Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and an NHS ester. This compound is engineered for efficient and selective bioconjugation, enabling the stable attachment of biomolecules under mild, aqueous conditions. It is an essential reagent for researchers and manufacturers in biopharmaceutical development, proteomics, and advanced material science, facilitating the creation of PEGylated therapeutics, diagnostic probes, and functionalized surfaces.

Application

• Bioconjugation and PEGylation: Site-specific conjugation of peptides, proteins, and antibodies via NHS ester chemistry for therapeutic and diagnostic applications.
• Click Chemistry: Utilizes the azide group for copper-catalyzed or strain-promoted azide-alkyne cycloaddition (CuAAC/SPAAC) to attach labels, drugs, or other functional groups.
• ADC (Antibody-Drug Conjugate) Development: Serves as a versatile linker for constructing homogeneous ADCs by connecting cytotoxic payloads to monoclonal antibodies.
• Surface Functionalization: Modification of nanoparticles, biosensors, and microarray surfaces to introduce bio-orthogonal handles for subsequent immobilization.
• Hydrogel Formation: Crosslinking agent for creating PEG-based hydrogels with tailored properties for tissue engineering and drug delivery systems.
• Diagnostic Reagent Synthesis: Production of fluorescent or radioactive probes for imaging and in vitro diagnostics.

Basic Information

Product Name	Azido-Peg24-Nhs Ester
CAS No.	2375600-46-5
Molecular Formula	C58H109N5O28
Molecular Weight	~1364.5 g/mol
Synonyms	NHS-PEG24-Azide; Azido-PEG24-NHS Ester; N-Hydroxysuccinimide Ester of Azido-PEG24-acid; α-Azido-ω-NHS-PEG24; PEG24 Azide NHS Ester; N3-PEG24-NHS; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate
EINECS	Contact for details

Quality Control

Our Azido-Peg24-Nhs Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, NMR for structural confirmation, and functional group analysis. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90% of theoretical
Functional Group Assay (NHS Ester)	≥ 90% of theoretical

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.