Description

Cyanine5.5 NHS Ester is a near-infrared fluorescent labeling reagent designed for the covalent conjugation of biomolecules. This compound is critical for creating stable, high-performance fluorescent probes and conjugates used in advanced bioanalytical applications. It is essential for researchers and manufacturers in the fields of biopharmaceutical development, in vivo imaging, and diagnostic assay production who require reliable, high-purity labeling agents.

Application

• Antibody and Protein Labeling: Conjugation to monoclonal antibodies, proteins, and peptides for fluorescence-activated cell sorting (FACS), immunohistochemistry (IHC), and Western blotting.
• Nucleic Acid Probes: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• In Vivo Imaging Agents: Development of targeted near-infrared (NIR) imaging probes for preclinical small animal studies due to its deep tissue penetration and minimal autofluorescence.
• Diagnostic Assay Development: Manufacturing of fluorescent lateral flow assays and microarrays for point-of-care and high-throughput screening.
• Nanoparticle Functionalization: Surface modification of liposomes, polymeric nanoparticles, and quantum dots for targeted drug delivery and imaging.
• Bioconjugation Research: A key reagent in academic and industrial R&D for exploring new biomolecular tagging strategies and biosensor development.

Basic Information

Product Name	Cyanine5.5 NHS Ester
CAS No.	2375105-86-3
Molecular Formula	C44H49N3O6
Molecular Weight	715.9 g/mol
Synonyms	Cyanine5.5 N-hydroxysuccinimide ester; Cy5.5 NHS ester; Sulfo-Cyanine5.5 NHS ester (if sulfonated); 1-(5-Carboxypentyl)-1'-methyl-3,3,3',3'-tetramethylindodicarbocyanine-5,5'-disulfonic acid NHS ester; NIR-783 NHS ester analog; Cyanine 5.5 carboxylic acid succinimidyl ester; Cy5.5 SE; Cy5.5-NHS
EINECS	Contact for details

Quality Control

Our Cyanine5.5 NHS Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and moisture content is provided with every shipment to ensure traceability and compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue to green solid
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	675 - 685 nm
Emission Maximum (λem)	695 - 705 nm
Extinction Coefficient (ε)	≥ 190,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.