Description

Dbco-Peg4-Triethoxysilane is a heterobifunctional crosslinking reagent that combines a bioorthogonal dibenzocyclooctyne (DBCO) group with a triethoxysilane anchor via a tetraethylene glycol (PEG4) spacer. This molecular architecture is critical for creating stable, site-specific conjugations between biomolecules and inorganic surfaces, particularly silicon-based materials. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, surface functionalization, and advanced material science, enabling the development of biosensors, diagnostic assays, and functionalized nanoparticles.

Application

• Surface Functionalization: Covalent immobilization of DBCO-labeled proteins, antibodies, or oligonucleotides onto silicon, glass, or silica surfaces for biosensor and microarray development.
• Bioconjugation Platform: Serves as a key linker for click chemistry-based (strain-promoted azide-alkyne cycloaddition, SPAAC) conjugation of azide-modified biomolecules to solid supports.
• Nanoparticle Modification: Functionalization of silica nanoparticles (SiNPs) or other oxide-based nanomaterials to create targeted drug delivery systems or imaging agents.
• Material Science: Incorporation into sol-gel formulations or polymer matrices to introduce bio-recognition elements for creating smart, responsive materials.
• Diagnostic Assay Development: Fabrication of stable capture surfaces in ELISA, lateral flow assays, or other diagnostic platforms requiring oriented and robust biomolecule attachment.
• Cell Culture & Tissue Engineering: Modification of glass or silicon-based substrates to create patterned surfaces for controlled cell adhesion and growth studies.

Basic Information

Product Name	Dbco-Peg4-Triethoxysilane
CAS No.	2353410-02-1
Molecular Formula	C31H41NO8Si
Molecular Weight	583.75 g/mol
Synonyms	DBCO-PEG4-Triethoxysilane; (Azadibenzocyclooctyne-PEG4)-Triethoxysilane; Dibenzocyclooctyne-PEG4-Triethoxysilane; DBCO-PEG4-Silane; 3-(2-(2-(2-(3-(1,5-Dihydro-2,4-diphenylbenzo[e][1,2,5]triazepin-3-yl)propanamido)ethoxy)ethoxy)ethoxy)propyltriethoxysilane; DBCO-PEG4-TES; DBCO-Silane Reagent; SPAAC Silane Coupling Agent
EINECS	Contact for details

Quality Control

Every batch of Dbco-Peg4-Triethoxysilane is manufactured under strict quality protocols and undergoes comprehensive analytical testing to ensure identity, purity, and performance consistency. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from HPLC, NMR (1H and 13C), and mass spectrometry analysis. Our quality management system supports compliance with relevant standards for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated at -20°C.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in DMSO, DMF, acetonitrile, THF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.