Description

Tco-Peg6-Nhs Ester CAS NO 2353409-96-6 is a heterobifunctional crosslinking reagent featuring a trans-cyclooctene (TCO) group for bioorthogonal click chemistry and an N-hydroxysuccinimide (NHS) ester for efficient amine conjugation. This compound is engineered to enable rapid, specific, and catalyst-free labeling of biomolecules, making it a critical tool for advanced bioconjugation workflows. It is essential for researchers and developers in the fields of biopharmaceuticals, diagnostic assay development, and chemical biology who require high-purity, reliable linkers for creating stable, functionalized conjugates.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via amine groups.
• Protein and Peptide Labeling: Functionalization of proteins, peptides, and other amine-containing biomolecules with TCO tags for subsequent tetrazine-based click reactions.
• Diagnostic Probe Synthesis: Construction of imaging probes and detection reagents for in vitro diagnostics (IVD) and preclinical imaging (e.g., PET, fluorescence).
• Surface Functionalization: Modification of nanoparticles, microspheres, biosensor chips, and other solid supports for biomolecule immobilization.
• Chemical Biology Research: As a key reagent for studying protein interactions, cellular trafficking, and biomolecular dynamics using bioorthogonal chemistry.
• Oligonucleotide Conjugation: For attaching targeting ligands, dyes, or other functional modules to oligonucleotides via amine-modified bases or linkers.

Basic Information

Product Name	Tco-Peg6-Nhs Ester
CAS No.	2353409-96-6
Molecular Formula	C₃₃H₅₄N₂O₁₂
Molecular Weight	662.8 g/mol
Synonyms	trans-Cyclooctene-PEG6-NHS ester; TCO-PEG6-NHS; (E)-Cyclooct-4-enyl 2,5,8,11,14,17-hexaoxanonadecan-19-oate 3,4-dihydro-2,5-dioxo-1H-pyrrole-1-propanoic acid ester; TCO-PEG6-Succinimidyl Ester; 2,5-Dioxopyrrolidin-1-yl 19-((1E,5E)-cyclooct-4-en-1-yloxy)-2,5,8,11,14,17-hexaoxanonadecanoate; TCO-PEG6-OSu; TCO hexaethylene glycol NHS ester
EINECS	Contact for details

Quality Control

Our Tco-Peg6-Nhs Ester is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity for sensitive research and development applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity assessment and NMR for structural confirmation. Certificates of Analysis (COA) detailing identity, purity, and impurity profiles are provided with every shipment to support your regulatory and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize condensation and hydrolysis of the active NHS ester group.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (H-NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.