Description

n-(Azido-Peg4)-n-Bis(Peg4-Nhs Ester) CAS NO 2353409-90-0 is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and two NHS ester termini. This compound is engineered for efficient and selective bioconjugation, enabling the stable coupling of biomolecules under mild, aqueous conditions. It is a critical reagent for researchers and developers in biopharmaceuticals, diagnostics, and advanced materials science who require precise and reliable crosslinking strategies.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific conjugation of cytotoxic payloads to monoclonal antibodies via click chemistry and amine coupling.
• PEGylation of Proteins and Peptides: To enhance solubility, stability, and circulation half-life of therapeutic biologics.
• Surface Functionalization: For modifying nanoparticles, biosensors, and microarray slides with bioactive ligands.
• Hydrogel Formation: As a crosslinking agent in the synthesis of biocompatible PEG-based hydrogels for tissue engineering and drug delivery.
• Diagnostic Assay Development: For creating stable conjugates between enzymes, fluorophores, or haptens and antibodies or other proteins.
• Biomaterial Fabrication: In the development of functionalized scaffolds and coatings for medical devices.

Basic Information

Item	Details
Product Name	n-(Azido-Peg4)-n-Bis(Peg4-Nhs Ester)
CAS No.	2353409-90-0
Molecular Formula	C45H74N6O23
Molecular Weight	1067.1 g/mol
Synonyms	Azido-PEG4-Bis(PEG4-NHS ester); NHS-PEG4-Azide-PEG4-NHS; α-Azido-ω-bis(N-hydroxysuccinimidyl ester) PEG; N3-PEG4-(NHS-PEG4)2; Heterobifunctional PEG Crosslinker, Azide/NHS; 2,5,8,11,14,17,20,23,26,29,32,35,38,41-Tetradecaoxa-44-azatetratetracontane-44,46-dioic acid, 45-azido-, 1,46-bis(2,5-dioxo-1-pyrrolidinyl) ester; PEG-based Click Chemistry Crosslinker
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg4)-n-Bis(Peg4-Nhs Ester) is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and functional group titration, to ensure consistent performance. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and the NHS ester groups are susceptible to hydrolysis. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (H-NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90% of theoretical
Functional Group Assay (NHS Ester)	≥ 85% of theoretical

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.