Description

Sulfo-Cyanine3 Azide is a near-infrared fluorescent dye derivative functionalized with a reactive azide group and water-solubilizing sulfonate moieties. This compound is essential for bioorthogonal labeling via copper-free click chemistry, enabling the tagging of biomolecules in live-cell imaging and diagnostic assays. It is a critical reagent for researchers and manufacturers in the fields of bioconjugation, flow cytometry, and in vivo imaging who require high-sensitivity, photostable, and water-compatible fluorescent probes.

Application

• Bioorthogonal Labeling: For copper-free click chemistry conjugation to alkyne-modified proteins, antibodies, nucleic acids, and other biomolecules.
• Live-Cell Imaging: Enables real-time tracking and visualization of cellular processes with minimal background and high photostability.
• Flow Cytometry & FACS: Used as a fluorescent tag for cell surface marker detection and cell sorting applications.
• In Vivo Imaging: Suitable for preclinical research due to its near-infrared emission, which offers deeper tissue penetration.
• Diagnostic Assay Development: A key component in the development of fluorescent immunoassays and lateral flow tests.
• Polymer & Materials Science: Used to fluorescently label synthetic polymers, nanoparticles, and hydrogels for tracking and characterization.

Basic Information

Product Name	Sulfo-Cyanine3 Azide
CAS No.	2322322-57-4
Molecular Formula	C37H42KN5O6S2
Molecular Weight	779.99 g/mol
Synonyms	Sulfo-Cy3 Azide; Sulfo Cy3 N3; Cyanine3 sulfonyl azide; 2-((1E,3E)-5-((E)-1-(5-azidopentyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate; Water-soluble Cy3 azide; NIR dye azide; Bioorthogonal fluorescent probe
EINECS	Contact for details

Quality Control

Our Sulfo-Cyanine3 Azide is manufactured under strict quality systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. Certificates of Analysis (COA) detailing purity, identity, and performance data are provided with every shipment to ensure compliance with research and diagnostic application standards.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is light-sensitive (store away from light) and hygroscopic. Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Extinction Coefficient	≥ 150,000 cm-1M-1 at λmax
Water Content (KF)	≤ 5.0%
Functional Performance	Conjugates efficiently with model alkyne substrates

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.