Description

2-(Azido-Peg3-Amido)-1,3-Bis(Nhs Ester) is a heterobifunctional crosslinking reagent featuring an azide group and two N-hydroxysuccinimide (NHS) ester groups, connected by a triethylene glycol (PEG3) spacer. This molecular architecture is critical for creating stable, site-specific conjugates between biomolecules, such as proteins and oligonucleotides, under mild aqueous conditions. It is an essential tool for researchers and developers in the fields of bioconjugation, proteomics, and the development of antibody-drug conjugates (ADCs) and diagnostic assays. The compound is supplied with a high level of purity to ensure reliable and reproducible coupling efficiency.

Application

• Bioconjugation and Crosslinking: Primary application for covalently linking proteins, peptides, antibodies, or other amine-containing molecules to azide-modified surfaces, polymers, or biomolecules via click chemistry.
• Antibody-Drug Conjugate (ADC) Development: Used as a linker-payload construct to attach cytotoxic drugs to monoclonal antibodies, leveraging the NHS esters for amine coupling and the azide for subsequent bioorthogonal reactions.
• Proteomics and Biomarker Discovery: Enables the labeling and enrichment of proteins for mass spectrometry analysis, facilitating the study of protein-protein interactions and post-translational modifications.
• Diagnostic Assay Development: Employed in the fabrication of biosensors and immunoassays by immobilizing capture antibodies or antigens onto solid supports functionalized with complementary click chemistry handles.
• Polymer and Material Science: Functionalization of polymers, nanoparticles, or surfaces with bioactive ligands to create smart materials for drug delivery or tissue engineering.
• Nucleic Acid Labeling: Conjugation of fluorescent dyes or other reporter molecules to amine-modified oligonucleotides for FISH, sequencing, or other genomic applications.

Basic Information

Product Name	2-(Azido-Peg3-Amido)-1,3-Bis(Nhs Ester)
CAS No.	2320560-36-7
Molecular Formula	C₂₄H₃₄N₆O₁₂
Molecular Weight	598.56 g/mol
Synonyms	2-(Azido-PEG3-amido)-1,3-bis(NHS ester); NHS-PEG3-Azide; Azido-PEG3-NHS ester; N3-PEG3-NHS; α-Azido-ω-NHS ester PEG3 crosslinker; 1,3-Bis(2,5-dioxopyrrolidin-1-yl) 2-(2-(2-(2-azidoethoxy)ethoxy)ethylamino)-2-oxoethyl propanedioate; Heterobifunctional PEG crosslinker, Azide-NHS
EINECS	Contact for details

Quality Control

Our 2-(Azido-Peg3-Amido)-1,3-Bis(Nhs Ester) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and NMR for structural confirmation, to ensure it meets the high standards required for sensitive research and development applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and the NHS ester groups are susceptible to hydrolysis. For long-term stability, store in aliquots under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.