Description

Cyanine7 DBCO is a near-infrared fluorescent dye functionalized with a dibenzocyclooctyne (DBCO) group for bioorthogonal click chemistry. This compound is essential for the sensitive, copper-free labeling and tracking of biomolecules in complex biological systems without interfering with native biochemical processes. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, bioimaging, and in vitro diagnostics for developing advanced probes and assays.

Application

• Bioorthogonal Labeling: Copper-free, strain-promoted azide-alkyne cycloaddition (SPAAC) with azide-modified proteins, antibodies, oligonucleotides, or small molecules.
• In Vivo & In Vitro Imaging: Conjugation to targeting agents (e.g., peptides, antibodies) for near-infrared fluorescence (NIRF) imaging in live cells and animal models.
• Diagnostic Probe Development: Synthesis of fluorescent probes and sensors for high-sensitivity detection in diagnostic assays.
• Polymer & Material Science: Fluorescent labeling of azide-functionalized polymers, nanoparticles, and surfaces for tracking and characterization.
• Flow Cytometry & Microscopy: Preparation of fluorescent conjugates for cell sorting, analysis, and high-resolution cellular imaging.
• Drug Delivery & Pharmacokinetics: Tracking the distribution, uptake, and clearance of azide-tagged drug carriers or therapeutic agents.

Basic Information

Product Name	Cyanine7 DBCO
CAS No.	2253710-45-9
Molecular Formula	C52H57N3O3S2+
Molecular Weight	852.17 g/mol
Synonyms	Cy7 DBCO; DBCO-Cy7; Sulfo-Cyanine7 DBCO (if sulfonated); DBCO-Cyanine7; 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-2-((1E,3E)-3-((1E,3E)-5-((E)-2-((E)-3,3-dimethyl-5-sulfo-1-(4-sulfobutyl)indolin-2-ylidene)ethylidene)-4-((E)-2-(dibenzo[b,f][1,2,3]triazolo[1,5-d][1,4]diazepin-12(6H)-yl)vinyl)cyclohex-2-en-1-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium-5-sulfonate (IUPAC); DBCO-Sulfo-Cy7; Near-IR DBCO Dye; DBCO-Cy7 fluorescent probe
EINECS	Contact for details

Quality Control

Our Cyanine7 DBCO is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via mass spectrometry and NMR, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and other critical parameters is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~747 nm ± 5 nm
Emission Maximum (λem)	~774 nm ± 5 nm
Extinction Coefficient (ε)	Contact for details
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF, water (sulfonated form)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.