Description

Cyanine3 Amine CAS NO 2247688-56-6 is a reactive amine-functionalized derivative of the popular cyanine dye family, designed for covalent conjugation to biomolecules. This compound is essential for creating highly fluorescent, stable probes for advanced bioanalytical and diagnostic applications. It is primarily used by researchers and manufacturers in the life sciences, pharmaceutical development, and in vitro diagnostics (IVD) sectors for labeling proteins, antibodies, peptides, and oligonucleotides.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to create bright, photostable probes for immunofluorescence (IF), flow cytometry, and Western blotting.
• Nucleic Acid Labeling: Modification of oligonucleotides, primers, and probes for fluorescence in situ hybridization (FISH), PCR, and microarray analysis.
• Bioconjugation for Drug Discovery: Tagging of small molecules, peptides, or nanoparticles for cellular uptake, binding affinity, and pharmacokinetic studies.
• In Vitro Diagnostic (IVD) Reagent Development: Manufacturing of fluorescent conjugates for clinical assay kits and point-of-care testing devices.
• Live-Cell and Tissue Imaging: Preparation of targeted imaging agents for confocal microscopy and intravital imaging due to its favorable spectral properties.
• Biomaterial and Surface Functionalization: Covalent attachment to polymers, gels, or sensor surfaces to create fluorescent biosensors or patterned substrates.

Basic Information

Product Name	Cyanine3 Amine
CAS No.	2247688-56-6
Molecular Formula	C34H39N3O2+ (as a salt)
Molecular Weight	~521.70 g/mol (free base form)
Synonyms	Cy3 Amine; Cy3-NH2; Cy3 Amine, SE equivalent precursor; 2-((1E,3E)-5-((E)-1-(6-Aminohexyl)-3,3-dimethylindolin-2-ylidene)penta-1,3-dien-1-yl)-3,3-dimethyl-1-(6-aminohexyl)-3H-indol-1-ium; Cyanine3 aliphatic amine; Amino-Cy3; Reactive Cy3 dye
EINECS	Contact for details

Quality Control

Our Cyanine3 Amine is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional validation for conjugation efficiency. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark green to blue solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	548 - 552 nm
Emission (λmax)	562 - 568 nm
Extinction Coefficient (ε)	~150,000 L·mol⁻¹·cm⁻¹
Solubility	Soluble in DMSO, DMF, methanol, acetonitrile
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.