Description

Sulfo Cy5 Maleimide is a high-performance, water-soluble fluorescent labeling reagent designed for the site-specific conjugation of biomolecules. Its core value lies in enabling the creation of bright, stable, and functional bioconjugates for advanced detection and imaging applications. This compound is essential for researchers and developers in the fields of bioconjugation, flow cytometry, in vivo imaging, and diagnostic assay development, where reliable and efficient labeling of proteins, antibodies, and peptides is required.

Application

• Site-Specific Protein & Antibody Labeling: Conjugation via cysteine residues to create fluorescently tagged proteins for immunoassays (ELISA, Western Blot) and flow cytometry.
• In Vivo Imaging Probe Development: Labeling of targeting molecules (e.g., peptides, antibodies) for near-infrared (NIR) fluorescence imaging in preclinical research.
• Diagnostic Kit Manufacturing: Production of fluorescent detection reagents for clinical diagnostics and point-of-care testing devices.
• Nucleic Acid Detection: Labeling of oligonucleotides or probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Biomolecular Interaction Studies: FRET (Förster Resonance Energy Transfer) pair construction for studying protein-protein interactions and conformational changes.
• Cell Tracking and Analysis: Long-term cell labeling and tracking in multiplexed cellular assays due to its bright fluorescence and photostability.

Basic Information

Product Name	Sulfo Cy5 Maleimide
CAS No.	2242791-82-6
Molecular Formula	C37H42KN3O10S2 (Potassium salt form)
Molecular Weight	~815.98 g/mol
Synonyms	Sulfo-Cyanine5 Maleimide; Sulfo-Cy5 Maleimide; Cyanine5 sulfonyl maleimide potassium salt; Sulfo-Cy5 Mal; 2,5-Pyrrolidinedione, 1-[6-[(1E,3E)-5-[[(1E,3E)-1-(1,3-dihydro-1,3-dioxo-2H-isoindol-2-yl)-3-sulfo-1,3-pentadien-1-yl]amino]-1,3,5-hexatrien-1-yl]-4-sulfo-1,3-cyclohexadien-1-yl]-, potassium salt (1:1); Water-soluble Cy5 Maleimide; NIR fluorescent maleimide dye
EINECS	Contact for details

Quality Control

Our Sulfo Cy5 Maleimide is manufactured under strict quality protocols to ensure batch-to-batch consistency and performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, mass spectrometry for identity confirmation, and spectrophotometry for labeling efficiency verification. A detailed Certificate of Analysis (COA) documenting purity, absorbance/emission profiles, and functional performance is provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to purple solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum (λem)	662 - 668 nm
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹
Solubility	Soluble in water, DMSO, DMF
Single Isomer Content (HPLC)	≥ 90.0%
Free Maleimide (HPLC)	≤ 2.0%
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.