Description

Sulfo-Cyanine3.5 NHS Ester is a water-soluble, amine-reactive fluorescent labeling reagent designed for the bioconjugation of proteins, peptides, antibodies, and other biomolecules. This compound is critical for creating stable, fluorescently tagged probes used in advanced bioanalytical and diagnostic applications. It is essential for researchers and manufacturers in the fields of biomedical research, in vitro diagnostics (IVD), and flow cytometry who require high-performance, near-infrared fluorescent labels with excellent aqueous solubility.

Application

• Antibody Labeling: Conjugation to monoclonal and polyclonal antibodies for use in immunofluorescence, immunohistochemistry (IHC), and fluorescence-activated cell sorting (FACS).
• Protein & Peptide Tagging: Fluorescent labeling of proteins, enzymes, and peptides for tracking, quantification, and interaction studies in biochemical assays.
• Nucleic Acid Detection Probes: Preparation of fluorescent oligonucleotide probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• In Vitro Diagnostic (IVD) Kits: Manufacturing key components for clinical diagnostic assays, including lateral flow tests and microarrays.
• Live-Cell Imaging: Labeling of biomolecules for non-invasive tracking and visualization in live-cell microscopy due to its favorable spectral properties.
• Nanoparticle Functionalization: Conjugation to the surface of nanoparticles for targeted imaging and drug delivery systems.

Basic Information

Product Name	Sulfo-Cyanine3.5 NHS Ester
CAS No.	2231670-91-8
Molecular Formula	C37H41KN4O9S2
Molecular Weight	813.06 g/mol
Synonyms	Sulfo-Cy3.5 NHS Ester; Cyanine3.5 sulfonyl NHS ester; Sulfo-Cyanine 3.5 N-hydroxysuccinimide ester; 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxo-1-(3-sulfonatopropyl)-1H-indol-2-yl)ethylidene)-2-((1E,3E)-3-(1-(6-(3-sulfonatopropylamino)-6-oxohexyl)-3,3-dimethyl-5-sulfo-2,3-dihydro-1H-indol-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium, potassium salt; Sulfo-Cy3.5 SE; NHS-Cy3.5 sulfonate
EINECS	Contact for details

Quality Control

Our Sulfo-Cyanine3.5 NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Extinction Coefficient	Contact for details
Fluorescence Emission	~ 590-610 nm
Water Content (KF)	≤ 2.0%
Single Spot (TLC)	One major spot

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.