Description

Cyanine3.5 NHS Ester is a reactive near-infrared fluorescent dye derivative designed for efficient labeling of biomolecules. This compound is crucial for researchers and developers requiring high-sensitivity detection and imaging in complex biological systems. It is primarily used by professionals in the biotechnology, pharmaceutical, and diagnostic industries for applications such as fluorescence microscopy, flow cytometry, and in vivo imaging.

Application

• Bioconjugation and Probe Development: Covalent labeling of proteins, antibodies, peptides, and oligonucleotides via primary amine groups.
• Bioimaging: Used as a fluorescent tag for near-infrared (NIR) fluorescence microscopy and super-resolution imaging techniques.
• Diagnostic Assays: Development of fluorescent immunoassays (FLISA) and lateral flow tests for high-sensitivity detection.
• Flow Cytometry: Cell labeling and tracking in immunophenotyping and cellular analysis.
• In Vivo Imaging: Conjugation to targeting molecules for non-invasive optical imaging in preclinical research.
• Drug Delivery & Pharmacokinetics: Fluorescent tagging of drug candidates or nanocarriers to study distribution and uptake.
• Biomolecular Interaction Studies: Förster Resonance Energy Transfer (FRET) and other proximity-based assays.

Basic Information

Product Name	Cyanine3.5 NHS Ester
CAS No.	2231670-85-0
Molecular Formula	C41H45N3O6S2+ • Anion-
Molecular Weight	Contact for details
Synonyms	Cy3.5 NHS Ester; Cyanine 3.5 N-hydroxysuccinimide ester; Sulfo-Cyanine3.5 NHS ester (if sulfonated); 1H-Benz[e]indolium, 2-[5-[1-(2,5-dioxo-1-pyrrolidinyl)oxy]-1-oxo-2,5-dihydro-1H-pyrrol-3-yl]-3,3-dimethyl-1-(3-sulfopropyl)-, inner salt (possible variant); N-Succinimidyl Cy3.5 ester; Near-IR fluorescent labeling reagent; Amine-reactive Cy3.5 dye
EINECS	Contact for details

Quality Control

Our Cyanine3.5 NHS Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science research. Certificates of Analysis (COA) detailing purity, absorbance, fluorescence, and functional performance are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark green to blue solid or film
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~591 nm
Emission Maximum (λem)	~604 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	< 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.